目的 研究药用植物石菖蒲内生真菌灰黄青霉Penicillium griseofulvum Z005的次级代谢产物。方法 利用包括硅胶、ODS、Sephadex LH-20、半制备HPLC等色谱方法，对目标菌株的大米固体发酵提取物进行系统的分离纯化，并结合理化常数与现代的谱学方法进行化合物的结构鉴定。结果 从石菖蒲内生真菌灰黄青霉Z005固体发酵产物中分离鉴定了1个新化合物和12个已知化合物。分别为灰黄青霉呋喃醇A（1）、chaetominine（2）、dehydrocyclopeptine（3）、1-methylcyclopenin（4）、4-(2-羟基丁炔氧基) 苯甲酸（5）、penipratynolene（6）、hypofuran B（7）、lobechine（8）、4-(2-甲酰基-5-甲氧基甲基-1H-吡咯-1-基) 丁酸（9）、2-呋喃甲醇-(5'→11)-1,3-环戊二烯-[5,4-c]-1H-邻二氮杂萘（10）、N-乙酰色胺（11）、1H-吲哚-3-基甲基氨基甲酸甲酯（12）和顺式阿魏酸甲酯（13）。结论 灰黄青霉Z005为首次从石菖蒲健康组织中分离获得，化合物1为新的苯并呋喃类化合物，命名为灰黄青霉呋喃醇A（penicifuranol A）；化合物2～10和12～13均为首次从该种真菌中分离得到。

Objective To study the secondary metabolites of an endophytic fungus Penicillium griseofulvum Z005 isolated from the medicinal plant Acorus tatarinowii. Methods Various column chromatography methods, including silica gel, ODS, Sephadex LH 20, and semipreparative HPLC were applied to isolate and purify compounds from the solid rice fermentation extract of P. griseofulvum Z005, and their chemical structures were elucidated by physical chemical constants and modern spectroscopy techniques. Resutls One new compound and 12 known compounds were isolated and identified from the solid fermentation products of endophytic fungus P. griseofulvum Z005, and their structures were identified as penicifuranol A (1)，chaetominine (2), dehydrocyclopeptine (3), 1-methylcyclopenin (4), 4-(2-hydroxybutynoxy)benzoic acid (5), penipratynolene (6), hypofuran B (7), lobechine (8), 4-(2-formyl-5-methoxymethyl-1H-pyrrole-1-yl)butanoic acid (9), 2-furanmethanol- (5'→11)- 1,3-cyclopentadiene-[5,4-c]-1H-cinnoline (10), N-acetyltryptamine (11), methyl 1H-indol-3-ylmethylcarbamate (12), and methyl (Z)-ferulic acid ester (13), respectively. Conclusion The endophytic fungus P. griseofulvum Z005 was firstly gained from the healthy tissue of A. tatarinowii, and compound 1 was a new benzofuran compound, and compounds 2 —10 and 12 —13 are isolated from fungus P. griseofulvum Z005 for the first time.

R284.1

河南省科技厅科技攻关项目（172102310430）