目的 通过降低提取温度改善土茯苓Smilacis Glabrae Rhizoma提取物及其配方颗粒的溶化性。方法 分别采用回流提取、煮开后80℃水浴浸提、直接80℃水浴浸提、煮开后70℃水浴浸提、直接70℃水浴浸提5种方法制备土茯苓提取物并对各提取物的溶化性进行分析,同时考察5种提取方法对土茯苓提取物的特征图谱、多糖与淀粉含量、出膏率、落新妇苷含量及转移率的影响。结果 上述5种方法制备的提取物中不溶物含量分别为34.93%、26.67%、24.48%、29.65%、4.03%,与回流提取相比其余4种提取方法均能改善土茯苓提取物的溶化性,其中直接70℃水浴浸提对溶化性改善最明显。不同提取方法制备的土茯苓提取物的特征图谱无明显差异,表明其质量稳定。相比于回流提取,直接70℃水浴浸提的土茯苓提取物的淀粉含量降低了11.74%;落新妇苷含量(38.6 mg/g)及其转移率最高(60.95%)。结论 采用70℃水浴浸提法可显著改善土茯苓配方颗粒的溶化性。

Objective To improve the solubility of Smilacis Glabrae Rhizoma (SGR) extract and its formulation granules by lowering the extraction temperature. Methods Reflux extraction, 80 ℃ water bath extraction after boiling, 80 ℃ water bath extraction, 70 ℃ water bath extraction after boiling, and 70 ℃ water bath extraction were used to prepare the extract of SGR, and the solubility of each extract was analyzed. At the same time, the effects of five extraction methods on the characteristic chromatograms, polysaccharide and starch contents, paste yield, content and transfer rate of astilbin were investigated. ResultsThe contents of insoluble matter in the extracts prepared by the above five methods was 34.93%, 26.67%, 24.48%, 29.65%, and 4.03%, respectively. The solubility of SGR extract could be improved by using the other four extraction methods compared to reflux extraction, with 70 ℃ water bath extraction showing the most obvious improvement. There was no significant difference in the characteristic chromatograms of the extracts prepared by different extraction methods, indicating that their quality was stable. Compared to reflux extraction, 70 ℃ water bath extraction showed an 11.74% reduction in starch content; astilbin content (38.6 mg/g) and its transfer rate (60.95%) were the highest. Conclusion The solubility of SGR formulation granules can be significantly improved by the method of 70 ℃ water bath extraction.

R283.6

国家中医药多学科交叉创新团队项目（ZYYCXTD-D-202209）；华润三九现代中药制药有限公司-成都中医药大学企业委托横向课题（3010210586）