目的 对黄芩Scutellaria baicalensis中化学成分及其血中移行成分进行定性分析,为黄芩药效物质基础研究提供参考。方法 采用U3000-Q Exactive超高效液相色谱-液质联用仪,获得黄芩水煎液、混合对照品溶液、空白血清和含药血清的液相色谱-质谱信息,通过比对OTCML(Thermo Science)、mzCloud、ChemSpider、Massbank数据库及部分对照品的提取离子流色谱图、质谱碎片裂解信息,鉴定出目标化合物。结果 黄芩水煎液中共鉴定出125个化合物,包括黄酮类、苯丙酸苷类、环烯醚萜苷类、三萜类、生物碱等;对黄芩血中移行成分进行分析,共鉴定出25个原型成分,包括黄酮类、二萜类、环烯醚萜类和生物碱类;其中19个化合物为首次报道的黄芩血中移行成分,包括半乳糖醛酸、α,α-海藻糖、黄芩前列素B、白杨素6-C-葡萄糖苷8-C-阿拉伯糖苷、ajugapitin、白杨素6-C-α-L-阿拉伯吡喃糖苷8-C-葡萄糖苷、圣草酚-7-葡糖苷、白杨素6-C-葡萄糖苷8-C-α-L-阿拉伯吡喃糖苷、白杨素6-C-阿拉伯糖苷8-C-葡萄糖苷、6-O-甲基黄芩苷、二氢黄芩苷、芹菜素7-O-葡萄糖醛酸、蓟黄素、5,7,2,-三羟基黄酮、5,8,2'-三羟基-6,7-二甲氧基黄酮、黄芩新素I、柳穿鱼黄素、二氢梓醇、香叶木素。结论 对黄芩水煎液中的化学成分进行了定性分析的基础上,明确了大鼠ig黄芩水煎液后血中移行成分,表明黄芩中具有潜在药理活性的成分主要为黄酮类化合物,为进一步阐明黄芩的药理作用和机制奠定基础。

Objective The chemical constituents in Scutellaria baicalensis and its absorbed prototypes components were qualitatively analyzed, which provided a reference for the basic research of the pharmacodynamics of Scutellaria baicalensis. Methods U3000-Q Exactive LC-MS/MS was used to obtain the liquid chromatography-mass spectrometry information of Scutellaria baicalensis aqueous decoction, mixed standard solution, blank serum and drug-containing serum, and then the target compound was identified by comparing the extracted ion chromatogram and mass spectrometry fragmentation information of OTCML (Thermo Science), mzCloud, ChemSpider, Massbank databases and some standards. Results 125 compounds were identified from the Scutellaria baicalensis aqueous decoction, including flavonoids, phenylpropionic glycosides, iridoid glycosides, triterpenoids, alkaloids, etc, among which 25 compounds were reported for the first time. In addition, the same analysis method was used to analyze the absorption components in the serum of Scutellaria baicalensis. 25 prototype compounds were identified, including flavonoids, diterpenoids, iridoid glycosides terpenoids and alkaloids, among which 19 compounds were reported for the first time, including galacturonic acid, α,α-trehalose, scutellaprostin B, chrysin 6-C-glucoside 8-C-arabinoside, ajugapitin, chrysin 6-C-α-L-arabinopyranoside- 8-C-glucoside, eriodictyol 7-glucuronide, chrysin 6-C-glucoside-8-C-α-L-arabinopyranoside, chrysin 6-C-arabinoside 8-C-glucoside, 6-O-methylscutellarin, dihydrobaicalin, apigenin 7-O-glucuronide, skrofulein, 5,7,2,-trihydroxyflavone, 5,8,2'-trihydroxy-6,7- dimethoxyflavone, skullcapflavone I, pectolinarigenin, dihydrocatalpol, diosmetin. Conclusion In this study, the chemical components in Scutellaria baicalensis aqueous were qualitatively analyzed, and on this basis, components with potential the transitional components in the blood of rats after ig Scutellaria baicalensis aqueous were identified, indicating that the chemical pharmacological activity in Scutellaria baicalensis were mainly flavonoids, which laid a foundation for further elucidating the pharmacological action and mechanism of Scutellaria baicalensis.

R284.1

佳木斯大学药学学科团队（JDXKTD-2019005）