目的 研究逍遥软胶囊中柴胡-白芍药对不同配伍比例HPLC指纹图谱与抗抑郁作用药效之间的相关性，明确柴胡与白芍配伍发挥疏肝解郁作用的潜在物质基础。方法 采用慢性束缚小鼠模型，以强迫游泳及悬尾不动时间为指标评价柴胡-白芍药对疏肝解郁的作用，利用偏最小二乘回归分析法进行谱-效关联分析，快速筛选出其药效贡献较大成分。结果 阳性药以及不同配伍比例柴胡-白芍药对均明显地缩短小鼠悬尾及强迫游泳不动时间，具有抗抑郁作用，其中以柴胡-白芍（2∶1）的抗抑郁效果最为显著。通过偏最小二乘回归分析发现，柴胡-白芍药对14个共有峰中7个特征峰与其抗抑郁作用显著相关且贡献较大。结论 通过谱效关系研究，推测柴胡配伍白芍发挥疏肝解郁的药效物质基础，可能为包括芍药内酯苷、芍药苷和柴胡皂苷a等在内的7个化合物，为其质量标志物的确定及质量控制提供了依据。

Objective To study the correlation between HPLC fingerprints of different compatibility proportions of Bupleuri Radix (BR)-Paeoniae Raidx Alba (PRA) herb pair of Xiaoyao Soft Capsule (XSC) and anti-depressant effect, and provide experimental basis for clarifying the potential material basis of BR-PRA herb pair for soothing liver and relieving depression. Methods HPLC method was used to establish the fingerprint chromatography of different compatibility proportions of BR-PRA herb pair. Chronic restraint stress (CRS) was applied to establish stress depression model and the depressive behaviors of mice were observed by forced swimming test (FST) and tail suspension test (TST). Partial least squares-discriminant (PLSR) analysis was applied to analyze the correlation, so as to quickly screen out the ingredients with greater contribution to efficacy. Results The immobility time in FST and TST was decreased significantly in all treatment groups, and positive drug, Sinisan and different compatibility proportions of BR-PRA herb pair had anti-depressant effect. Among them, the ratio 2:1 of BR-PRA herb pair had the strongest anti-depressant effect. PLSR analysis showed that seven characteristic peaks from 14 shared peaks of BR-PRA herb pair were significantly correlated with the anti-depressant effect and had greater contribution to efficacy. Conclusion Through the spectrum-effect study, it is speculated that the effective components of BR-PRA herb pair for soothing liver-qi stagnation may be the seven compounds including alibiflorin, paeoniflorin, saikosaponin a, etc, which provide a basis for the determination of quality markers and quality control.

R283.6

国家重点研发计划（2018YFC1706903）；陕西省中药基础与新药研究重点实验室基金（19JS019）；陕西省自然科学基金（2022JM-451）；陕西省高校科协青年人才托举计划项目（20160228）；陕西中医药大学学科创新团队项目（2019-YL11）