[关键词]
[摘要]
目的 建立金银花和山银花指纹图谱,对河南封丘金银花与湖南不同基原山银花进行区别比较,并对封丘不同品种金银花进行质量评价。方法 利用HPLC法构建金银花和山银花药材的指纹图谱;利用“中药色谱指纹图谱相似度评价系统(2012版)”进行相似度评价、以“SIMCA13.0”软件进行主成分分析(principal component analysis,PCA)分析;通过HPLC法测定河南封丘不同品种金银花成分含量。结果 河南封丘产金银花相似度均在0.95以上,湖南各基原山银花相似度在0.97以上,金银花和山银花相似度在0.57~0.79,豫金二号与金银花及山银花药材相似度均在0.6以下,共有多成分PCA分析也能显著区分金银花和山银花。金银花各品种中北花1号酚酸类以绿原酸、异绿原酸A和异绿原酸C之和为5.1%,比其他品种高约15%;木犀草苷豫金1号含0.062%、北花1号0.064%,比四季花高约25%、比羊角花高约50%。结论 该化学指纹图谱方法能显著区分金银花和山银花,二者间的化学成分差异亦能为金银花指标性成分的选择提供参考。金银花不同栽培品种成分含量差异明显,豫金1号和北花1号药材质量较优,是具有进一步开发利用的潜在优质种质资源。
[Key word]
[Abstract]
Objective To establish the HPLC fingerprint of Jinyinhua (Lonicerae Japonicae Flos) and Shanyinhua (Lonicerae Flos). The differences of Lonicerae Japonicae Flos in Fengqiu of Henan Province and Lonicerae Flos in Hunan Province were compared based on the HPLC fingerprint and the quality of different varieties of Lonicerae Japonicae Flos in Fengqiu was evaluated. Methods Fingerprints of Lonicerae Japonicae Flos and Lonicerae Flos were establishd by HPLC. The Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2012 version) was used for similarity evaluation, and the PCA analysis was performed by (SIMCA13.0). HPLC was used to detect the content of different varieties of Lonicerae Japonicae Flos in Henan. Results The similarity of all varieties of Lonicerae Japonicae Flos in Fengqiu was above 0.95, similarity of Lonicerae Flos from different origins was above 0.97. The similarity of Lonicerae Japonicae Flos and Lonicerae Flos was 0.57-0.79. The similarity between the Yujin No.2 and Lonicerae Japonicae Flos and Lonicerae Flos were less than 0.6. PCA analysis can distinguish Lonicerae Japonicae Flos and Lonicerae Flos significantly. The total contents of chlorogenic acid, isochlorogenic acid A and isochlorogenic acid C in the Beihua No.1 was 5.1%, which was 15% higher than other varieties of Lonicerae Japonicae Flos. The content of luteoloside in the Yujin No.1 was 0.062% and in the Beihua No. 1 was 0.064%, which were about 25% higher than that in the Sijihua and about 50% higher than that in the Yangjiaohua. Conclusion The HPLC fingerprint can significantly distinguish Lonicerae Japonicae Flos and Lonicerae Flos, and the difference of chemical components between them could also provide reference for the selection of Q-markers of Lonicerae Japonicae Flos. The content of components in different varieties of Lonicerae japonicae Flos was significantly different. The Yujin No.1 and the Beihua No.1 have better quality, which were potential germplasm resources for further development and utilization.
[中图分类号]
R286.2
[基金项目]
国家自然科学基金资助项目(81603232)