目的 建立山慈菇的HPLC指纹图谱，对其特征成分进行含量测定并结合化学计量学分析方法分析不同商品规格山慈菇（冰球子、毛慈菇）化学成分的差异，为山慈菇药材的质量控制提供参考。方法 优化山慈菇药材的提取方法，应用HPLC法分别建立冰球子和毛慈菇指纹图谱，并对特征成分进行含量测定。采用相似度评价、聚类分析（cluster analysis，CA）、主成分分析（principalcomponent analysis，PCA）、正交偏最小二乘法判别分析（orthogonal partial least squares discriminant analysis，OPLS-DA）、Fisher线性判别分析（fisher linear discrimination analysis，FLDA）对特征成分进行数据分析。结果 建立了冰球子和毛慈菇HPLC指纹图谱，分别匹配了46个和43个共有峰，共标示出17个色谱峰，指认出天麻素、loroglossin、dactylorhin A、白芨苷、山药素III、白芨联菲A、白芨联菲B、卷瓣兰蒽8个色谱峰并同时建立了上述除天麻素以外7个成分的HPLC含量测定方法。CA与PCA结果可将2种不同商品规格山慈菇样品明显区分。OPLS-DA分析结合含量测定Fisher判别分析结果表明，2种不同商品规格的山慈菇样品间存在显著性差异的5种成分是loroglossin、dactylorhin A、白芨苷、白芨联菲B、卷瓣兰蒽（P＜0.001）。结论 所建立的HPLC指纹图谱结合CA、PCA、OPLS-DA分析及多成分含量测定能客观、全面、有效地确定不同商品规格山慈菇中主要成分的差异，可为山慈菇质量评价体系的完善提供依据。

Objective To establish the HPLC fingerprint of Cremastrae Pseudobulbus Pleiones Pseudobulbus (Shancigu), determine the content of its characteristic components, and analyze the difference of the chemical components of the different commercial specifications (Bingqiuzi and Maocigu) of Shancigu combined with a variety of chemometrics analyses to provide a reference for its quality control. Methods The extraction method of Shancigu was optimized. HPLC was used to establish the fingerprint of Bingqiuzi and Maocigu respectively, and the content of characteristic components was determined. The data of characteristic components were analyzed by similarity evaluation, cluster analysis (CA), principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and Fisher linear discriminant analysis (FLDA). Results HPLC fingerprints of Bingqiuzi and Maocigu were established, and a total of 46 and 43 common peaks were matched in Bingqiuzi and Maocigu respectively, 17 chromatographic peaks were calibrated, and eight chromatographic peaks were identified, including gastrodin, loroglossin, dactylorhin A, militarine, batatasin III, blestriarene A, blestriarene B, monbarbatain A, and HPLC method for the determination of the above seven components except gastrodin was established simultaneously. The results of CA and PCA can distinguish two kinds of samples of different commercial specifications. The results of OPLS-DA analysis combined with content determination Fisher discriminant analysis showed that loroglossin, dactylorhin A, militarine, blestriarene B, and monbarbatain A (P < 0.001) were the components with significant differences between the samples of two different commodity specifications. Conclusion The HPLC fingerprint of Shancigu combined with CA, PCA, OPLS-DA analysis and multi-component content determination, can determine the differences of the main components in different commercial specifications of Shancigu objectively, comprehensively and effectively, and provide a basis for the improvement of the quality evaluation system of Shancigu.

R286.2

国家自然科学基金资助项目（82073971）