[关键词]
[摘要]
目的 研究兔耳风属植物杏香兔耳风Ainsliaea fragrans的化学成分。方法 采用溶剂萃取、大孔吸附树脂、硅胶、ODS柱色谱以及HPLC等技术进行化学成分分离纯化,运用核磁共振等波谱数据分析鉴定化合物结构。结果 从杏香兔耳风乙醇提取物中分离得到20个化合物,分别鉴定为3β-O-β-D-glucopyranosyl-8α-hydroxy-11α,13-dihydrozaluzanin C(1)、岩白菜素(2)、glucozaluzanin C(3)、芹菜素-7-O-β-D-葡萄糖醛酸甲酯(4)、costunolide-15-β-D-glucopyranoside(5)、芹菜素-7-O-β-D-葡萄糖醛酸苷(6)、木犀草素-7-O-β-D-葡萄糖醛酸苷(7)、芹菜素-6,8-二-C-葡萄糖苷(8)、绿原酸(9)、1,3-O-二咖啡酰奎宁酸(10)、3-O-反式对羟基香豆酰奎宁酸(11)、木犀草素-7-O-β-D-葡萄糖苷(12)、木犀草素(13)、原儿茶酸(14)、5-O-顺式对羟基香豆酰奎宁酸(15)、1-O-咖啡酰奎宁酸(16)、隐绿原酸(17)、5-O-反式对羟基香豆酰奎宁酸(18)、3,5-O-二咖啡酰奎宁酸(19)、2′-O-E-caffeoyl-8α-hydroxy-11α,13-dihydro-3β-O-β-D-glucozaluzanin C(20)。结论 化合物2、4~8、11、15~16、18为首次从兔耳风属植物中分离得到。
[Key word]
[Abstract]
Objective To investigate chemical constitutes of Ainsliaea fragrans. Methods The compounds were isolated and purified by solvent extraction, macroporous adsorbent resin, silica gel column chromatography, ODS and semi-preparative HPLC. The structures of the compounds were elucidated by spectral analyses, including MS and NMR spectral techniques. Results Twenty compounds were isolated from A. fragrans and were identified as 3β-O-β-D-glucopyranosyl-8α-hydroxy-11α,13- dihydrozaluzanin C (1), bergenin monohydrate (2), glucozaluzanin C (3), apigenin 7-O-β-(6′′-methylglucuronide) (4), costunolide-15-β-D-glucopyranoside (5), apigenin-7-O-β-D-glucuronopyranoside (6), luteolin-7-O-β-D-glucuronopyranoside (7), apigenin-6,8-di-C-β-D-glucopyranoside (8), chlorogenic acid (9), 1,3-di-O-caffeoylquinic acid (10), 3-p-O-trans-coumaroylquinic acid (11), luteolin-7-O-β-D-glucoside (12), luteolin (13), protocatechuic acid (14), 5-p-cis-coumaroylquinic acid (15), 1-O- caffeoylquinic acid (16), cryptochlorogenic acid (17), 5-p-trans-coumaroylquinic acid (18), 3,5-O-dicaffeoylquinic acid (19), and 2′-O-E-caffeoyl-8α-hydroxy-11α,13-dihydro-3β-O-β-D-glucozaluzanin C (20). Conclusion Compounds 2, 4—8, 11, 15—16 and 18 were isolated from the genus Ainsliaea for the first time.
[中图分类号]
R284.1
[基金项目]
国家自然科学基金资助项目(82173945);国家自然科学基金资助项目(81760702);国家自然科学基金资助项目(81560636);江西省教育厅科学技术研究项目(GJJ211261);江西省食品监督管理局科研项目(2020JS10);江西省卫生健康委员会科技计划项目(202211429);江西省中医药管理局科技计划项目(2022A366);江西中医药大学创新基金项目(JZYC22S60);江西中医药大学校级科技创新团队发展计划(CXTD22001)