目的 对多刺绿绒蒿Meconopsis horridula MYB转录因子家族的鉴定分析，为多刺绿绒蒿MYB转录因子的生物学功能研究提供理论依据。方法 基于转录组测序数据，利用生物信息学方法对多刺绿绒蒿MYB转录因子家族成员进行鉴定，并对其理化性质、二级结构、系统进化、保守基序和表达模式进行分析。结果 多刺绿绒蒿中共鉴定到106个MYB转录因子，包括49个1R-MYB、52个R2R3-MYB、5个3R-MYB转录因子。多刺绿绒蒿MYB转录因子氨基酸数目为72～976，蛋白相对分子质量为8750～110 680，等电点为4.720～10.452，不稳定系数为28.348～71.011。MYB转录因子均为亲水性蛋白质，亚细胞定位预测主要在细胞核中，蛋白二级结构以α-螺旋和无规则卷曲为主。系统发育分析显示多刺绿绒蒿R2R3-MYB分为24个亚组，其中有18个亚组能分别与拟南芥各亚组聚类。106个MYB转录因子中，28个转录因子的表达量随海拔升高而升高，11个转录因子表达量随海拔升高而降低。结论 首次对多刺绿绒蒿MYB转录因子家族进行了鉴定分析，为深入研究MYB转录因子在多刺绿绒蒿生长发育中的作用和应对高原极端环境的调控机制提供了基础数据和理论依据。

Objective To identify and analyze MYB transcription factor family of Meconopsis horridula, so as to provide a theoretical basis for biological function research.Methods Based on RNA sequencing data, the MYB transcription factor family of M. horridula was identified and classified by bioinformatics methods. The physicochemical properties, secondary structure, phylogeny, conserved motifs and expression patterns of MYB transcription factors were analyzed. Results A total of 106 MYB transcription factor family members from M. horridula RNA sequencing data were identified, including 49 1R-MYB, 52 R2R3-MYB, and five 3R-MYB. The amino acid number, molecular weight, isoelectric point and instability coefficient of MYB transcription factor of M. horridula ranged from 72 to 976, 8 750 to 110 680 4.720 to 10.452, 28.348 to 71.011. The 106 MYB transcription factors were hydrophilic proteins, and the prediction of subcellular location was mainly in the nucleus. The secondary structure of the protein was mainly α-helix and random coil. Phylogenetic analysis showed that R2R3-MYB of M. horridula was divided into 24 subgroups, of which 18 subgroups were clustered with each subgroup of Arabidopsis thaliana. Among the 106 MYB transcription factors, the expression levels of 28 transcription factors were increased with elevation, and 11 transcription factors were decreased with elevation.Conclusion In this study, the MYB transcription factor family of M. horridula was identified and analyzed based on RNA sequencing data, which provided a basic data for growing development and regulatory mechanisms to cope with the extreme environment in the plateau of MYB transcription factors family in M. horridula.

R282.12

西藏自治区科技厅中央引导地方项目（XZ202001YD0028C，XZ202102YD0031C）；西藏大学研究生“高水平人才培养计划”（2020-GSP-S051）