目的 对当归Angelica sinensis MADS-box基因家族进行筛选与生物信息学分析，并对SOC1-4基因进行克隆与表达验证。方法 基于当归全长转录组筛选MADS-box基因家族，利用在线工具进行生物信息学分析，并利用qRT-PCR检测SOC1-4基因在不同材料中的表达水平。结果 当归全长转录组中含有29个MADS-box基因家族成员，可分为10个亚家族，共含有6个保守基序，蛋白质序列长度为49～422 aa，相对分子质量为5 697.56～49 624.90，等电点为5.06～11.00，亚细胞结果显示当归MADS-box基因家族成员分别定位于叶绿体、细胞核、细胞质和线粒体，二级结构预测及3D建模显示同一亚家族结构相似；SOC1-4基因克隆片段与全长转录组测序结果一致；SOC1-4基因随种苗春化作用和植株发育时期延长呈现高表达，在抽薹植株、茎和叶中高表达，而在冷冻规避春化作用种苗中呈现低表达。结论 当归含有29个MADS-box成员，各成员理化性质和结构存在一定的差异，SOC1-4基因表达水平与当归抽薹开花生理调控一致，为当归MADS-box基因家族调控抽薹开花相关研究提供借鉴。

Objective To screen and analyze MADS-box gene family bioinformatics of Angelica sinensis, clone the SOC1-4 gene and perform bioinformatic analysis. Methods The MADS-box gene family was screened based on the full-length transcriptomes of A. sinensis, the bioinformatics was analyzed using the online tools, and the expression levels of SOC1-4 gene in different materials were validated by qRT-PCR. Results A total of 29 MADS-box gene family members were identified from A. sinensis, with 10 subfamily classified and six motifs included; the sequence length of MADS-box proteins ranged from 49 to 422 aa, the relative molecular mass ranges from 5 697.56 to 49 624.90, and the isoelectric point ranged from 5.06 to 11.00; the sub-cellular results showed that the members of the A. sinensis MADS-box gene family was located in the chloroplast, nucleus, cytoplasm and mitochondria; and secondary structure prediction and 3D modeling indicated that there was a similar structure for the same subfamily. The cloned fragment of the SOC1-4 gene was consistent with the full-length transcript. The SOC1-4 gene were over-expressed with the extension of seedlings vernalization and plants development, as well as in bolted plants, stems and leaves, respectively; while down-regulated in the seedlings with frozen-avoided vernalization. Conclusion There are 29 MADS-box gene family members in A. sinensis, and certain differences exist in physical and chemical properties and structure among the 29 members; the expression levels of SOC1-4 gene are in accordance with the physiological regulation of bolting and flowering of A. sinensis, The results will provide theoretical basis of MADS-box gene family for regulating bolting and flowering of A. sinensis.

R286.12

国家自然科学基金项目（32160083）；干旱生境作物学国家重点实验室（甘肃农业大学）基金项目（GSCS-2021-Z03）；道地药材生态种植与质量保障项目（202103003）；财政部和农业农村部：国家现代农业产业技术体系（CARS-21）