目的 对花椒Zanthoxylum bungeanum果皮正丁醇部分化学成分进行研究。方法 利用大孔树脂柱色谱、硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱、半制备HPLC等方法进行分离纯化，并综合应用HR-ESI-MS、NMR、IR、CD等多种波谱方法确定化合物结构。结果 从花椒果皮正丁醇部分分离得到25个化合物，分别鉴定为2α,3β-dihydroxy-3α-vanilloylmethyl-γ-lactone（1）、phellolactone（2）、香草酸（3）、异香草酸（4）、4-hydroxycryptone（5）、ZP-amide A（6）、ZP-amide B（7）、ZP-amide D（8）、马齿苋酰胺E（9）佛手柑内酯（10）、(±)-dorsteniol（11）、苔黑酚葡萄糖苷（12）、phloroacetophenone 4-O-β-D-glucopyranoside（13）、(1R,2R,4S)-trans-2-hydroxy-1,8-cineole-2-O-β-D-glucopyranoside（14）、(3S,6S)-6-ethenyltetrahydro-2,2,6-trimethyl-2H-pyran-3-yl-6-O-β-D-glucopyranoside（15）、trans-linalool-3,6-oxide-7-O-β-D-glucopyranoside（16）、betulabuside A（17）、大豆苷（18）、葛根素（19）、金丝桃苷（20）、槲皮素-3-O-β-D-葡萄糖苷（21）、槲皮素-3-O-β-D-鼠李糖苷（22）、扁蓄苷（23）、橙皮苷（24）和tamarixetin 7-rutinoside（25）。结论 化合物1为新的γ-内酯类化合物，命名为异花椒戊内酯（isophellolactone），化合物1、2、9、11、13、14、17～19和22、23系首次从花椒属植物中分离得到，化合物5、10、15和24系首次从花椒中分离得到。

Objective To investigate the chemical constituents of pericarps of Huajiao (Zanthoxylum bungeanum). Method The n-butanol fraction of pericarps of Z. bungeanum was isolated and purified by macroporous resin column chromatography, silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-preparative HPLC. And their structures were elucidated by various spectroscopic methods, such as HR-ESI-MS, NMR, IR, CD, et al. Result A total of 25 compounds were isolated from the n-butanol fraction of pericarps of Z. bungeanum. They were identified as 2α,3β-dihydroxy-3α-vanilloylmethyl-γ-lactone (1), phellolactone (2), vanillic acid (3), isovanillic acid (4), 4-hydroxycryptone (5), ZP-amide A (6), ZP-amide B (7), ZP-amide D (8), oleracein E (9), bergapten (10), (±)-dorsteniol (11), orcinol glucoside (12), phloroacetophenone 4-O-β-D-glucopyranoside (13), (1R,2R,4S)-trans-2-hydroxy-1,8-cineole-2-O-β-D-glucopyranoside (14), (3S,6S)-6-ethenyltetrahydro-2,2,6-trimethyl-2H-pyran-3-yl-6-O-β-D-glucopyranoside (15), trans-linalool-3,6-oxide-7-O-β-D-glucopyranoside (16), betulabuside A (17), daidzin (18), puerarin (19), hyperin (20), quercetin 3-O-β-D-glucopyranoside (21), quercetin 3-O-β-D-rhamnoside (22), avicularin (23), hesperidine (24) and tamarixetin 7-rutinoside (25). Conclusion Compound 1 is obtained as a new γ-lactone named as isophellolactone. Compounds 1—2, 9, 11, 13—14, 17—19 and 22—23 are isolated from Zanthoxylum for the first time. Compounds 5, 10, 15 and 24 are isolated from Z. bungeanum for the first time.

R284.1

四川省中医药管理局2020年度花椒药用价值研发专项（2020HJZX002）；中央级公益性科研院所基本科研业务费专项资金项目（562018Y-5983）；国家市场监管总局科技计划项目（2019MK117）