目的 研究补肺活血胶囊（Bufei Huoxue Capsules，BHC）在大鼠血浆、胆汁、尿液、粪便中的原型成分及其代谢产物，归纳总结不同类型化合物的体内代谢规律。方法 大鼠ig BHC后，收集血浆、胆汁、尿液和粪便样品并进行预处理，采用超高效液相色谱-飞行时间质谱（ultra-high performance liquid chromatography-time-of-flight mass spectrometry，UPLC-Q-TOF-MS）及质量亏损过滤技术，分别在正、负离子模式下对原型成分及代谢产物进行分析鉴定。结果 共检测到没食子酸、芍药苷、毛蕊异黄酮、新补骨脂异黄酮、(S)-异补骨脂二氢黄酮、补骨脂苷等原型成分及代谢产物67个，其中酚酸类成分10个、单萜类成分7个、黄酮类成分35个、香豆素类成分12个、皂苷类成分3个，主要代谢途径包括氧化、还原、甲基化、葡糖醛酸结合、硫酸结合等。结论 初步阐明了BHC大鼠体内的代谢产物及其代谢转化特征，为基于体内过程揭示其药效物质基础奠定了基础，为其临床安全用药提供了科学依据。

Objective To study the prototype components and metabolites of Bufei Huoxue Capsules (补肺活血胶囊, BHC) in plasma, bile, urine and feces of rats, and summarize the metabolic rules of different types of compounds in vivo. Methods After ig BHC, the plasma, bile, urine and feces samples of rats were collected and pretreated. The prototype components and metabolites were analyzed and identified in positive and negative ion modes by ultra-high performance liquid chromatography-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with mass loss filtration techniques. Results A total of 67 prototypes and metabolites were detected, such as gallic acid, paeoniflorin, calycosin, neobavaisoflavone, isobavachin and psoralenoside, etc. Among them, there were 10 phenolic acids, seven monoterpenes, 35 flavonoids, 12 coumarins and three saponins. The main metabolic pathways included oxidation, reduction, methylation, glucuronic acid binding, sulfuric acid binding and so on. Conclusion This study preliminarily clarified the metabolites and transformation characteristics of BHC in rats, which laid a foundation for revealing its pharmacodynamic substance basis based on in vivo process, and provided a scientific basis for its safe clinical use.

R284.1

国家自然科学基金项目（81873189）；江苏省高校自然科学研究重大项目（18KJA360006）；江苏省“六大人才”高峰项目（YY-026）；江苏省研究生科研与实践创新计划项目（KYCX21_1728）