目的 研究栽培奇楠沉香Aquilariae Lignum Resinatum的化学成分及其抗炎活性。方法 采用硅胶、Sephadex LH-20凝胶等多种柱色谱分离技术分离化合物，并通过质谱、核磁共振等现代波谱学方法鉴定其结构，采用Griess法测试化合物的抗炎活性。结果 从栽培奇楠沉香的乙醇提取物中分离得到17个化合物，分别为2-[(4-甲氧基苯甲酰氧基)甲基]色酮（1）、2-(2-苯乙基)色酮（2）、2-[2-(3-羟基-4-甲氧基苯)乙基]色酮（3）、2-[2-(3-甲氧基-4-羟基苯)乙基]色酮（4）、2-[2-(4-甲氧基苯)乙基]色酮（5）、2-[2-(2-羟基苯)乙基]色酮（6）、2-[2-(4-羟基苯)乙基]色酮（7）、6-羟基-2-[2-(3-羟基-4-甲氧基苯)乙基]色酮（8）、6-甲氧基-2-[2-(4-甲氧基苯)乙基)]色酮（9）、(R)-2-[2-羟基-2-(4-羟基苯)乙基]色酮（10）、(S)-2-[2-羟基-2-(4-羟基苯)乙基]色酮（11）、4-羟基苯甲酸甲酯（12）、4-丙烯基苯甲醚（13）、7b-H-9(10)-烯-11,12-环氧-8-氧艾里莫芬烷（14）、(5S,7S,9S,10S)-(−)-9-羟基-芹子烷-3,11-二烯-14-醛（15）、β-榄香烯-9β-醇（16）、角鲨烯（17）。结论 化合物1为新化合物，化合物16首次在沉香中发现。体外抗炎活性测试结果表明，化合物8具有抑制脂多糖诱导小鼠单核巨噬细胞RAW264.7产生NO的活性，半数抑制浓度（median inhibition concentration，IC₅₀）值为（27.81±2.34）μmol/L。

Objective To investigate the chemical constituents from cultivated “Qi-Nan” agarwood and their anti-inflammatory activity. Methods Various chromatographic techniques, such as Sephadex LH-20 and silica gel were used for separation. Their structures were elucidated based on mass spectrometry (MS), nuclear magnetic resonance (NMR) and other modern spectroscopic methods. The anti-inflammatory activity of these compounds was screened by Griess method. Results Seventeen compounds were isolated from ethanol extract of cultivated “Qi-Nan” agarwood, which were 2-[(4-methoxybenzoyloxy)methyl]chromone (1), 2-(2-phenylethyl)chromone (2), 2-[2-(3-hydroxy-4-methoxyphenyl)ethyl]chromone (3), 2-[2-(3-methoxy-4-hydroxyphenyl)ethyl] chromone (4), 2-[2-(4-methoxyphenyl)ethyl]chromone (5), 2-[2-(2-hydroxyphenyl)ethyl]chromone (6), 2-[2-(4-hydroxyphenyl)ethyl]chromone (7), 6-hydroxy-2-[2-(3-hydroxy-4-methoxyphenyl)ethyl]chromone (8), 6-methoxy-2-[2-(4-methoxyphenyl)ethyl] chromone (9), (R)-2-[2-hydroxy-2-(4-hydroxyphenyl)ethyl]chromone (10), (S)-2-[2-hydroxy-2-(4-hydroxyphenyl)ethyl]chromone (11), methyl 4-hydroxybenzoate (12), anethole (13), 7β-H-9(10)-ene-11,12-epoxy-8-oxoeremophilane (14), (5S,7S,9S,10S)-(−)-9- hydroxy-selina-3,11-dien-14-al (15), β-elemen-9β-ol (16), squalene (17). Conclusion Compound 1 was a new compound, compound 16 was identified from agarwood for the first time. In vitro anti-inflammatory assay results showed that compound 8 inhibits the NO production in mouse mononuclear macrophages RAW264.7 induced by LPS with IC₅₀ value of (27.81 ±2.34) μmol/L.

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海南省重大科技计划项目（ZDKJ2021031）；海南省院士创新平台科研项目资金（YSPTZX202037）；财政部和农业农村部国家现代农业产业技术体系（CARS-21）；中央级公益性科研院所基本科研业务费专项（1630052022031，1630052022022）