目的 建立广山楂Malus doumeri指纹图谱，测定各批次广山楂的“活血化瘀”药效学指标，探讨广山楂化学成分与活血化瘀功效的谱效关系。方法 采用HPLC法建立18批广山楂的指纹图谱。利用大鼠高脂模型，测定不同批次广山楂的“活血化瘀”药效学指标，包括高密度脂蛋白胆固醇(high density lipoprotein cholesterol，HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterin，LDL-C)、总胆固醇(total cholesterol，TC)、三酰甘油(triglyceride，TG)含量及血液流变学指标等。运用偏最小二乘回归法(partial least-square method regression，PLSR)、灰色关联度分析法，分析广山楂化学成分与活血化瘀药效指标间的谱效关系。结果 构建了广山楂的HPLC图谱，确定了6个共有峰，并指认了其中的2个共有峰(绿原酸、根皮苷)。活血化瘀实验表明，不同批次广山楂活血药效指标间具有明显差异，在标定的广山楂6个特征峰中，峰2～6与HDL-C呈正相关，峰1、5、6与LDL-C呈正相关，峰1、5与TC呈正相关，峰3、4与TG呈正相关；灰色关联度分析表明，峰3、5、6所代表的化学成分与活血化瘀药效具有一定关联性。结论 建立了广山楂药材的指纹图谱，并对其谱效关系进行了研究，为广山楂的临床合理使用及质量控制研究提供了一定的参考依据。

Objective To establish the fingerprints of Malus doumeri, determine their pharmacodynamic indexes of blood-activating and stasis-resolving of various batches of M. doumeri, and explore the spectrum-effect relationship between the chemical components of M. doumeri and the efficacy of blood-activating and stasis-resolving. Methods The HPLC fingerprints of 18 batches of M. doumeri were established. The pharmacodynamic indexes of blood-activating and stasis-resolving of different batches of M. doumeri were determined using rat hyperlipidemia model, including high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), serum total cholesterol (TC) and triglyceride (TG), hemorheology index and so on. The partial least-square method regression (PLSR) and grey correlation analysis method was used to explore the spectrum-effect relationship between the chemical components of M. doumeri and the pharmacodynamic indexes of blood-activating and stasis-resolving. Results HPLC chromatogram of M. doumeri was established, six common peaks were identified, and two common peaks (chlorogenic acid and phloridzin) were calibrated. The experiment of blood-activating and stasis-resolving showed that there were significant differences in pharmacodynamic indexes among different batches of M. doumeri. Among the six characteristic peaks of M. doumeri, peaks 2—6 were positively correlated with HDL-C, peaks 1, 5 and 6 were positively correlated with LDL-C, peaks 1 and 5 were positively correlated with TC, and peaks 3 and 4 were positively correlated with TG; Grey correlation analysis showed that the chemical constituents represented by peak 3, 5 and 6 had a certain correlation with the efficacy of blood-activating and stasis-resolving. Conclusion In this experiment, the fingerprint of M. doumeri was established, and its spectrum-effect relationship was studied, which provided a certain reference basis for the clinical rational use and quality control of M. doumeri.

R285

国家重点研发计划课题：20种彝、傣、壮、瑶、苗民族药品种整理与质量评价(2019YFC1712304)