目的 研究补骨脂对不同肾虚证模型大鼠肝脏功能的影响。方法 将大鼠分为空白组、肾阳虚组和肾阴虚组，采用连续15 d ip氢化可的松(10 mg/kg)建立大鼠肾阳虚模型，连续10 d ig甲状腺素(80 mg/kg)联合利血平(0.5 mg/kg)建立大鼠肾阴虚模型。通过对大鼠行为学指标的观察以及血清睾酮(testosterone，T)、甲状腺素(thyroxine，T4)含量的检测，判断模型是否建立成功。验证造模成功后，各组大鼠给予不同剂量的补骨脂水煎液，15 d后通过测定血清生化指标丙氨酸氨基转移酶(alanine aminotransferase，ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase，AST)、碱性磷酸酶(alkaline phosphatase，ALP)、总胆固醇(total cholesterol，TC)、三酰甘油(triglyceride，TG)、白蛋白(albumin，ALB)和总蛋白(total protein，TP)的含量变化，结合肝脏病理组织观察综合评价补骨脂对不同组别大鼠肝脏功能的影响。结果 与肾阳虚组比较，肾阳虚补骨脂低剂量组和高剂量组ALT、AST活性及TC水平均有下降趋势，ALP活性及TG水平出现显著性下降(P＜0.01、0.001)，TP有上升趋势，肾阳虚补骨脂低剂量组ALB显著升高(P＜0.05)。与肾阴虚组比较，肾阴虚补骨脂低剂量组和高剂量组AST活性显著升高(P＜0.05)，ALB显著下降(P＜0.05、0.01)，补骨脂高剂量组ALT活性显著上升(P＜0.01)，TP显著下降(P＜0.05)。给予补骨脂后，肾阳虚组大鼠肝组织损伤程度减弱，结构趋于正常，而肾阴虚组出现肝脏病变现象。结论 补骨脂肝毒性与不同肾虚证候之间具有一定的关联性，其在肾阴虚证模型大鼠产生肝毒性。

Objective To study the effect of Buguzhi (Psoraleae Fructus) on liver function of rats with different kidney deficiency syndromes. Methods The rats were divided into blank group, kidney-yang deficiency group and kidney-yin deficiency group. The kidney-yang deficiency model was established by ip hydrocortisone (10 mg/kg) for 15 d, and kidney-yin deficiency model was established by ig thyroxine (80 mg/kg) combined with reserpine (0.5 mg/kg) for 10 d. Whether the model was established successfully was determined by observing the behavioral indexes of rats and detecting the contents of serum testosterone (T) and thyroxine (T4) in rats. After the model test was successful, rats were given different doses of Psoraleae Fructus decoction. After 15 d, by measuring the changes of serum biochemical indicators alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total cholesterol (TC), triglyceride (TG), albumin (ALB) and total protein (TP), to comprehensive evaluate the effect of Psoraleae Fructus on liver function of rats in different groups by observing liver pathological. Results Compared with kidney-yang deficiency group, ALT, AST activities and TC level in low-and high-dose Psoraleae Fructus groups of kidney-yang deficiency had a downward trend, ALP activity and TG level had a significant decline (P＜0.01, 0.001), TP had an upward trend, and ALB in low-dose Psoraleae Fructus group of kidney-yang deficiency had a significant increase (P＜0.05). Compared with kidney-yang deficiency group, AST activity in low-and high-dose Psoraleae Fructus groups with kidney-yin deficiency were significantly increased (P＜0.05), and ALB was significantly decreased (P＜0.05, 0.01); ALT activity in high-dose Psoraleae Fructus group was significantly increased (P＜0.01), TP was significantly decreased (P＜0.05). After administration of Psoraleae Fructus, degree of liver damage in kidney-yang deficiency group was weakened, and structure tended to be normal, while kidney-yin deficiency group showed liver lesions. Conclusion Psoraleae Fructus hepatotoxicity has certain correlation with different kidney deficiency syndromes, and it produces hepatotoxicity in kidney-yin deficiency model rats.

R285.5

国家自然科学基金资助项目(82004093)