目的 基于代谢组学技术探讨鳖血柴胡炮制前后“物质-效应”变化，阐释炮制对其“清肝退热”作用的影响。方法 通过中药非靶标代谢组学结合网络药理学分析，阐释物质基础变化与“清肝退热”作用相关性；通过2,4-二硝基苯酚致发热大鼠模型及肝脏代谢组学评价鳖血柴胡的解热作用，阐释效应变化与“清肝退热”作用相关性。结果 鳖血柴胡炮制前后的物质基础变化，在中医证候学上更侧重影响肝经，与风热、火热、湿热、血热等热证呈现证素相关性。解热药效学显示鳖血柴胡具有与生柴胡相似的解热作用，并对发热过程中的肝脏代谢物的影响较显著，具有肝脏损伤保护的优势。结论 “物质-效应”的代谢组学变化显示，炮制可促进鳖血柴胡“清肝退热”的综合作用。

Objective To explore the changes of “substance-effect” before and after processing of Chaihu (Bupleuri Radix) processed with Trionyx’s blood for the sake of illustrating the effect of processing on its efficiency of “liver-clearing and fever-relieving” based on metabolomics. Methods Non-target metabolomics and network pharmacology were used to analyze the correlation between changes of material basis and the effect of “liver-clearing and fever-relieving” of Bupleuri Radix processed with Trionyx’s blood. The antipyretic effect of Bupleuri Radix processed with Trionyx’s blood was evaluated by 2,4-dinitrophenol-induced fever rats and metabolomics of liver, which in order to interpret the correlation between the pharmacodynamic mechanism and the efficiency of “liver-clearing and fever-relieving” of Bupleuri Radix processed with Trionyx’s blood. Results The changes of material basis of Bupleuri Radix processed with Trionyx’s blood before and after processing were focused on affecting the liver meridian in TCM syndromes, which was related to syndrome elements with wind-heat, fire-heat, damp-heat, blood-heat, therefore corresponding efficiency was “liver-clearing and fever-relieving”. Antipyretic pharmacodynamics showed that Bupleuri Radix processed with Trionyx’s blood had a similar effect on Bupleuri Radix, and it had a significant effect on the metabolites in a fever, and it had the advantage of liver damage protection. Conclusion The metabolomic changes of “substance-effect” show that processing can promote the comprehensive effect of “liver-clearing and fever-relieving” of Bupleuri Radix processed with Trionyx’s blood.

R285.5

甘肃省中医药研究中心2019-2020年度开放课题立项(zyzx-2020-19);教育部第二批高校思想政治工作精品项目(教思政司函[2019]2号);第四次全国中药资源普查甘肃省试点工作中医药公共卫生专项(财社[2011]76号)