目的 建立芪冬颐心口服液的HPLC指纹图谱，结合UPLC-Q-TOF-MS方法对其成分进行归属，为芪冬颐心口服液质量控制提供理论依据。方法 建立10批次芪冬颐心口服液指纹图谱，采用“中药指纹图谱相似度评价系统（A版）”进行相似度评价，方法学考察精密度、重复性、稳定性，同时应用UPLC-Q-TOF-MS方法对其成分进行鉴别和归属。结果 10批次芪冬颐心口服液指纹图谱中有7个共有峰，指纹图谱相似度大于0.99。同时应用UPLC-Q-TOF-MS方法，正离子质谱数据鉴定出25个成分，负离子质谱数据鉴定出17个成分，正、负离子质谱数据共同鉴定出9个成分，并对每个共有峰进行单味药材归属。结论 HPLC指纹图谱结合UPLC-Q-TOF-MS方法准确可靠，重复性和稳定性较好，可为芪冬颐心口服液的质量控制提供参考。

Objective The HPLC fingerprint of Qidong Yixin Oral Liquid (芪冬颐心口服液) was established, and its components were assigned by UPLC-Q-TOF-MS method, providing theoretical basis for quality control of Qidong Yixin Oral Liquid. Methods The fingerprint of 10 batches of Qidong Yixin Oral Liquid was established, and the Similarity evaluation system of traditional Chinese medicine fingerprint (Version A) was used to evaluate the accuracy, repeatability and stability of the methodology. At the same time, the UPLC-Q-TOF-MS method was used to identify and classify its components. Results There were 7 common peaks in the fingerprint of 10 batches of Qidong Yixin Oral Liquid, and the similarity of fingerprint of 10 batches of Qidong Yixin Oral Liquid was greater than 0.99. At the same time, UPLC-Q-TOF-MS method was used to identify 25 components from positive ion mass spectrometry data, 17 components from negative ion mass spectrometry data, and 9 components from positive and negative ion mass spectrometry data, and each common peak was classified as a single medicinal material. Conclusion HPLC fingerprint combined with UPLC-Q-TOF-MS method is accurate, reliable, reproducible and stable, which can provide reference for quality control of Qidong Yixin Oral Liquid.

R284.1

吉林省科技发展计划项目(20200708039YY);中央支持地方改革发展基金资助项目(2020YQ12);黑龙江省自然科学基金资助项目(YQ2020H024)