目的 研究汉麻Cannabis sativa根部的化学成分。方法 采用硅胶柱色谱及HPLC等色谱技术进行分离纯化，通过理化性质与波谱数据分析鉴定化合物的结构。结果 从汉麻根甲醇提取液正己烷和醋酸乙酯萃取物中分离得到17个化合物，分别鉴定为(7S,8E)-3-羟基-1,3,5,8,10-没药烷五烯-9,12-内脂（1）、木栓酮（2）、β-谷甾醇（3）、齐墩果酸（4）、苯甲酸（5）、对羟基苯甲醛（6）、香草醛（7）、对羟基苯甲酸（8）、香草酸（9）、2α-羟基齐墩果酸（10）、顺式阿魏酸酰对羟基苯乙胺（11）、反式阿魏酸酰对羟基苯乙胺（12）、N-反式对羟基肉桂酰基对羟基苯乙胺（13）、2,5-二叔丁基苯酚（14）、(E)-N-(2-hydroxy-2-(4-hydroxyphenyl)ethyl)-3-(4-hydroxyphenyl)acrylamide（15）、(^S)-N-[2-hydroxy-2-(4-hydroxyphenyl)ethyl]-3-(4-hydroxy-3-methoxyphenyl)acrylamide（16）、9S,12S,13S-三羟基-10E,15Z-十八碳二烯酸（17）。结论 化合物1为新化合物，命名为汉麻根素；化合物4～9、11～14、16、17为首次从汉麻根中分离得到。化合物10、15为首次从该属植物中分离得到。

Objective To study the chemical constituents from the root of Cannabis sativa. Methods Chemical constituents were isolated by silica gel column chromatography and HPLC. The structures of these compounds were identified by spectral data and physicochemical properties analysis. Results A total of 17 compounds were separated from the root of C. sativa, which were identified as (7S,8E)-3-hydroxy-1,3,5,8,10-penten-9,12-bisabolanolide (1), friedelin (2), β-sitosterol (3), oleanolic acid (4), benzoic acid (5), p-hydroxybenzaldehyde (6), vanillin (7), p-hydroxybenzaldehyde (8), vanillic acid (9), 2α-hydroxyoleanolic acid (10), N-(cis-teruloyl) tyramine (11), N-(trans-teruloyl) tyramine (12), N-(trans-p-coumaroyl) tyramine (13), 2,5-di-tert-butylphenol (14), (E)-N-(2-hydroxy-2-(4-hydroxyphenyl) ethyl)-3-(4-hydroxyphenyl)acrylamide (15), (S)-N-[2-hydroxy-2-(4-hydroxyphenyl) ethyl]-3-(4-hydroxy-3-methoxyphenyl)-acrylamide (16), 9S,12S,13S-trihydroxy-10,15-octadecadienoic acid (17). Conclusion Compound 1 is new natural products named cannabisnoid, compounds 4―9, 11―14, 16, 17 are isolated from the root of C.sativa for the first time, compounds 10, 15 are isolated from Cannabis for the first time.

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黑龙江省省属高等学校基本科研业务费科研项目(135209218);齐齐哈尔市科技局重点公关项目(ZDGG-202002);齐齐哈尔大学研究生创新科研项目(YJSCX2021003);中央支持地方高校改革发展资金人才培养项目