目的 探究肝癌患者的细胞焦亡基因与预后的相关性以及细胞焦亡差异基因的相关性，并预测调控差异表达基因的中药，通过分析中药的性味归经、药物分类探讨治疗肝癌患者细胞焦亡的中医理论，为中医药现代化提供新思路。方法下载TCGA数据库中肝癌患者的转录组数据，以关键词“pyroptosis”搜索GenCards数据库，获取细胞焦亡相关基因并筛选、分析其在肝癌的表达量、共表达情况，并进行预后分析、基因本体论（gene ontology，GO）功能富集分析、DO（diease ontology）疾病分析；再通过CTD数据库查找对基因有调控作用的化学成分。最后应用COREMINE数据库查找相关中药，并统计高频中药的性味归经、药物分类。结果 共筛选出16个与预后相关的细胞焦亡基因，其中11个基因有较多相关的中药，其中高频中药为茶树根、郁金、人参、丁香、干姜等；药性以寒、温为主；药味以苦、甘为主；归经以肝、肺经居多；以补虚药、清热药、化痰止咳平喘药、解表药、理气药和活血化瘀药为主。结论 通过生物信息学技术分析得出肝癌相关细胞焦亡基因及对应的高频中药，为治疗肝癌的中药新药开发及中医药理论的微观挖掘提供思路。

Objective To explore the correlation between cell pyroptosis gene and prognosis in patients with liver cancer, as well as the correlation of differential genes of cell pyroptosis, and to predict the traditional Chinese medicine for regulating differential expression genes. The traditional Chinese medicine theory for the treatment of cell pyroptosis in patients with liver cancer was discussed through the nature, taste and meridian attribution of traditional Chinese medicine and drug classification. To provide new ideas for modernization of traditional Chinese medicine.Methods The transcriptome data of liver cancer patients were downloaded from the TCGA database, and the keywords “pyroptosis” were used to search the GenCards database to obtain pyroptosis related genes, screen and analyze their expression levels and co-expression in liver cancer, and perform prognostic analysis, gene ontology (GO) functional enrichment analysis, DO (disease ontology) disease analysis; Then CTD database was used to find the chemical components that had regulatory effects on genes. Finally, the COREMINE database was used to find traditional Chinese medicines, and the the nature, taste, meridian attribution and classification of traditional Chinese medicines were counted.Results A total of 16 cell pyroptosis genes related to prognosis were screened out, of which 11 genes were more corresponding to traditional Chinese medicine. The high-frequency traditional Chinese medicine was obtained as follows: Chashugen (root of Camellia sinensis), Yujin (Curcumae Radix), Renshen (Ginseng Radix et Rhizoma), Dingxiang (Caryophylli Flos), Ganjiang (Zingiberis Rhizoma), etc. The medicinal properties were mainly cold and warm; Medicine taste were mainly bitter and sweet; Liver and lung meridian were the main channels. It was mainly used as medicine for tonifying deficiency, clearing heat, reducing phlegm, relieving cough and antiasthmatic, relieving surface, regulating qi and promoting blood circulation and removing blood stasis.Conclusion Through the analysis of bioinformatics technology, the pyrodeath genes related to liver cancer and the corresponding high-frequency traditional Chinese medicine were obtained, providing ideas for the development of new traditional Chinese medicine for the treatment of liver cancer and the micromining of traditional Chinese medicine theory.

R285

国家中医临床研究基地业务建设项目第二批科研专项(JDZX2015183);湖南省自然科学基金(2022JJ30458);长沙市自然科学基金(kq2202451)