目的 研究荆防颗粒对寒冷刺激诱导小鼠机体损伤的保护作用及其作用机制。方法 雄性昆明小鼠随机分为对照组、模型组和荆防颗粒高、中、低剂量（16、8、4g/kg），每组8只。给予相应药物连续干预7d，同时每天进行冰水浴寒冷刺激以建立小鼠机体损伤模型，每天测定小鼠体质量，实验结束后测定并计算脏器指数；观察小鼠耳组织外观和病理损伤程度；检测检测血清中丙氨酸氨基转移酶（alanine aminotransferase，ALT）、天冬氨酸氨基转移酶（aspartate aminotransferase，AST）活性及三酰甘油（triglycerides，TG）、肌酐（creatinine，CRE）、尿素氮（blood urea nitrogen，BUN）、白细胞介素-6（interleukin-6，IL-6）、IL-1β、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）水平；检测肌肉组织中抗冻蛋白（antifreeze protein，AFP）水平。结果 经荆防颗粒干预后，各组小鼠体质量无显著性差异，体质量变化较为平稳，其中荆防颗粒低剂量组小鼠体质量增长较快，脏器指数无显著差异；耳组织外观及病理切片结果显示，小鼠受寒冷刺激诱导的损伤程度减轻；血清中IL-6、IL-1β、TNF-α和BUN水平降低（P＜0.05、0.01）；肌肉组织中AFP水平升高（P＜0.05、0.01）。结论 荆防颗粒对寒冷刺激诱导的机体损伤具有保护作用，其作用机制与抑制炎症反应、增加AFP水平有关。

Objective To study the protective effect and mechanism of Jingfang Granules (荆防颗粒) on body injury induced by cold stimulation in mice.Methods Male Kunming mice were randomly divided into control group, model group and Jingfang Granules high-, medium- and low-dose (16, 8, 4 g/kg) groups, with eight mice in each group. Mice were given corresponding drugs for 7 d, and body injury model was established by ice water bath for cold stimulation every day. Body weight of mice was measured every day, and organ index was measured and calculated after the experiment; The appearance and pathological damage of ear tissue were observed; The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and levels of triglycerides (TG), creatinine (CRE), blood urea nitrogen (BUN), interleukin-6 (IL-6), IL-1β, tumor necrosis factor-α (TNF-α) in serum were detected; The level of antifreeze protein (AFP) in muscle tissue was detected.Results After Jingfang Granules intervention, there was no significant difference in body weight of mice in each group, and body weight change was relatively stable. The body weight of mice in Jingfang Granules low- dose group was rapidly increased, and organ index had no significant difference; The results of ear tissue appearance and pathological section showed that the degree of injury induced by cold stimulation in mice was reduced; IL-6, IL-1β, TNF-α and BUN levels in serum were decreased (P<0.05, 0.01); The level of AFP in muscle tissue was increased (P<0.05, 0.01).Conclusion Jingfang Granules has protective effect on body injury induced by cold stimulation, and its mechanism is related to inhibition of inflammatory reaction and increasing of AFP level.

R285.5

荆防颗粒二次开发研究项目(2180071720113)