目的 对缬草Valeriana officinalis水提取物中的化学成分进行研究。方法 运用正相硅胶柱色谱、ODS反相柱色谱、制备高效液相色谱等分离手段进行分离纯化，采用核磁共振、质谱等多种波谱学技术鉴定化合物的化学结构，并采用Griess法评价其对脂多糖诱导的小胶质细胞BV-2神经炎症的抑制活性。结果 从缬草水提取物中分离得到了12个化合物，分别为tinnevellin-8-O-β-D-glucopyranoside（1）、大黄素-8-O-β-D-吡喃葡萄糖苷（2）、(2R,3S)-肥牛木素（3）、左旋橄榄素-9'-O-β-D-吡喃葡萄糖苷（4）、左旋马尾松树脂醇-3a-O-β-D-吡喃葡萄糖苷（5）、金色酰胺醇乙酸酯（6）、松脂素（7）、8-羟基松脂素（8）、松脂素-4-O-β-D-吡喃葡萄糖苷（9）、8,8'-二羟基松脂素-4-O-β-D-吡喃葡萄糖苷（10）、(2S)-橙皮苷（11）和香蜂草苷（12）。结论化合物1～4和6均为首次从缬草属植物中分离得到。其中，化合物3～4、6、9、11～12在不影响BV-2的细胞活力前提下，可以抑制脂多糖诱导的BV-2细胞神经炎性因子一氧化氮（NO）的产生。

Objective To study the chemical constituents of the water-soluble extract of Valeriana officinalis.Methods Silica gel and ODS column chromatography, preparative high-performance liquid chromatography were applied for isolation of constituents from the water-soluble extract. NMR, MS and other related spectroscopic analyses were used to identify their structures, and a Griess method was used to evaluate their inhibitory activity on lipopolysaccharide-induced inflammation of BV-2 cells.Results Twelve compounds were isolated and identified as tinnevellin-8-O-β-D-glucopyranoside (1), emodin-8-O-β-D-glucopyranoside (2), (2R,3S)-ceplignan (3), (-)-olivil-9'-O-β-D-glucopyranoside (4), (-)-massoniresinol-3a-O-β-D-glucopyranoside (5), aurantiamide acetate (6), pinoresinol (7), 8-hydroxypinoresinol (8), pinoresinol-4-O-β-D-glucopyranoside (9), prinsepiol-4-O-β-Dglucopyranoside (10), (2S)-hesperidin (11) and didymin (12).Conclusion Compounds 1-4 and 6 are firstly isolated from the genus Valeriana. Compound 3-4、 6、 9、 11-12 showed inhibition on the production of neuroinflammatory factor NO induced by LPS in BV-2 cells, without any detectable influence on the cell viabilities.

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国家重点研发计划(2018YFC1707904);国家“重大新药创制”科技重大专项(2018ZX09735-002);天津市中央引导地方科技发展专项(21ZYJDJC00080)