目的 研究华南忍冬Lonicera confusa干燥花蕾（山银花）的化学成分。方法 综合应用HP-20、硅胶、ODS、Sephadex LH-20和半制备液相等多种色谱学方法进行分离纯化，并根据化合物的理化性质和核磁共振波谱数据进行结构鉴定。采用LPS诱导小鼠巨噬细胞RAW264.7模型，以抑制炎性细胞因子一氧化氮（NO）的分泌为评价指标对分离得到的化合物进行体外抗炎活性评价。结果 从华南忍冬干燥花蕾70%乙醇提取物中分离鉴定了5个化合物，分别为8-[α-L-arabinopyranosyl-(1''→6')-β-D-glucopyranosyl]-2,6-dimethyloct-1,2''-lactone（1）、天师酸（2）、黄麻脂肪酸F（3）、天师酸甲酯（4）和黄麻脂肪酸F甲酯（5）。结论 化合物1为新的单萜苷类化合物，命名为忍冬单萜A，化合物2～5为首次从忍冬属植物中分离得到。化合物对LPS诱导的RAW264.7细胞中NO的分泌均无显著的抑制作用。

Objective To study the constituents from the dried flower buds of Lonicera confusa.Methods The compounds were isolated and purified by column chromatography of HP-20 macroporous resin, silica gel, ODS, Sephadex LH-20, and semi-preparative RP-HPLC. Their structures were elucidated by physicochemical properties and spectral analyses. Taking NO as the evaluating indicator, the model of LPS-induced RAW264.7 cells was used to evaluate the in vitro anti-inflammatory activity of isolates.Results Five compounds were isolated and identified from the 70% ethanol extract of L. confusa, including 8-[α-L-arabinopyranosyl-(1''→6')-β-D-glucopyranosyl]-2,6-dimethyloct-1,2''-lactone (1), tianshic acid (2), corchorifatty acid F (3)、 tianshic acid methyl ester (4) and corchorifatty acid F methyl ester (5), respectively.Conclusion Compound 1 is a new monoterpene glycoside, named comfusanoside A. Compounds 2-5 are identified from Lonicera for the first time. All the isolates had no significant inhibitory effect on NO secretion in RAW264.7 cells stimulated by LPS.

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国家中医药管理局中央财政转移支付地方项目:基于重点研究室研究领域的中医药多学科研究能力提升项目-中药提取精制新技术