目的 研究人参固本口服液对椎间盘退变小鼠肠道菌群的影响。方法 28只小鼠随机分为假手术组、模型组、布洛芬(90mg/kg)组和人参固本口服液(3mL/kg),每组7只。采用尾部全层穿刺法制备椎间盘退变模型,给予相应药物干预28d,拍摄尾部核磁共振;采用苏木素-伊红(HE)、番红固绿染色观察小鼠肝、肾、椎间盘的病理变化;采用16SrRNA高通量测序检测小鼠结肠内肠道菌群组成和结构。结果 与假手术组比较,模型组椎间盘结构明显破坏;肠道菌群α多样性分析Chao指数、Shannon指数无明显差异,β多样性PC1、PC2水平均有明显偏移;疣微菌门(Verrucomicrobiota)、巴氏杆菌门(Patescibacteria)、杜氏杆菌属Dubosiella、阿克曼氏菌属Akkermansia的相对丰度显著降低(P<0.05、0.01),粪杆菌属Faecalibaculum相对丰度显著升高(P<0.001)。与模型组比较,人参固本口服液组失序的椎间盘结构得到修复;肠道菌群α多样性Chao指数、Shannon指数显著升高(P<0.05),β多样性在PC1水平更趋向于假手术组;疣微菌门、Lachnospiraceae_NK4A136_group、阿克曼氏菌属、回肠杆菌属Ileibacterium相对丰度显著升高(P<0.05、0.01、0.001),粪杆菌属相对丰度显著降低(P<0.001)。结论 人参固本口服液能够修复髓核、纤维环的结构,减轻炎性细胞浸润,延缓椎间盘退变的病理过程,其机制可能与调控肠道菌群微环境有关。

Objective To study the effect of Ginseng Guben Oral Liquid (人参固本口服液) on intestinal flora of mice with intervertebral disc degeneration (IVDD). Methods Twenty-eight mice were randomly divided into sham operation group, model group, ibuprofen (90 mg/kg) group and Ginseng Guben Oral Liquid (3 mL/kg), with seven mice in each group. The model of IVDD was made by tail full-thickness puncture, and corresponding drugs were given for 28 d, tail nuclear magnetic resonance was taken; HE and saffron fast green staining were used to observe the pathological changes of liver, kidney and intervertebral disc in mice; 16S rRNA high-throughput sequencing was used to detect the composition and structure of intestinal flora in colon of mice. Results Compared with sham operation group, intervertebral disc structure in model group was obviously destroyed; There was no significant difference in Chao index and Shannon index of α diversity analysis in intestinal flora, but PC1 and PC2 levels of β diversity were obviously deviated; Relative abundance of Verrucomicrobiota, Patescibacteria, Dubosiella and Akkermansia were significantly decreased (P< 0.05, 0.01), while Faecalibacterium relative abundance was significantly increased (P< 0.001). Compared with model group, disordered intervertebral disc structure in Ginseng Guben Oral Liquid group was repaired; Chao index and Shannon index of α diversity analysis in intestinal flora were significantly increased (P< 0.05), β diversity tended to sham-operation group at PC1 level; Relative abundance of Verrucomicrobiota, Lachnospiraceae_NK4A136_group, Akkermansia and Ileibacterium were significantly increased (P< 0.05, 0.01, 0.001), while relative abundance of Faecalibaculum was significantly decreased (P< 0.001). Conclusion Ginseng Guben Oral Liquid can repair the structure of nucleus pulposus and annulus fibrosus, reduce the infiltration of inflammatory cells and delay the pathological process of IVDD, and its mechanism may be related to the regulation of intestinal flora microenvironment.

R285.5

中药制药共性技术国家重点实验室开放基金资助项目（202009）