目的 探索外源过氧化氢（hydrogen peroxide，H₂O₂）预处理对垂盆草Sedum sarmentosum抗逆性及药材产量和品质的影响，为逆境信号物质诱导药材高产优质的栽培模式提供依据。方法 垂盆草叶面喷施不同浓度（0、1、10、100 mmol/L） H₂O₂后经历自然低温，收获时测定生长指标、生物量、多种黄酮类成分含量及多种体外抗氧化能力。结果 在低温条件下，喷施H₂O₂均显著提高垂盆草最大分枝长、叶片层数、分枝数、生物量、多种黄酮类成分含量以及体外抗氧化能力。随外源H₂O₂浓度提高，垂盆草叶片层数、分枝数、新生芽数、生物量及过氧化物酶（peroxidase，POD）酶活性呈下降趋势，而超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）酶活性及丙二醛（malondialdehyde，MDA）含量呈上升趋势。1、10 mmol/L H₂O₂处理的生物量分别比对照提高209.38%和87.50%。总黄酮、总酚酸含量以及槲皮素、山柰酚、异鼠李素含量均以10 mmol/L H₂O₂处理最高，比对照提高16.67%~37.84%，1 mmol/L H₂O₂处理次之。1 mmol/L H₂O₂处理垂盆草1，1-二苯基-2-三硝基苯肼（1，1-diphenyl-2-picrylhydrazyl，DPPH）和2，2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐[2，2'-azino-bis （3-ehtylbenzothiazolin-6-sulfnic acid），ABTS]自由基清除能力最强，而羟基自由基清除能力和抗脂质过氧化能力以10 mmol/L H₂O₂处理最强。结论 在低温条件下H₂O₂预处理有利于提高垂盆草抗寒性及药材产量和品质；综合比较生物量及活性成分含量，1或10 mmol/L H₂O₂预处理利于提高垂盆草抗寒性，高产优质兼得。

Objective To explore the effects of exogenous hydrogen peroxide (H₂O₂) pretreatment on the adversity resistance and the yield and quality of Sedum sarmentosum, and to provide a basis for the high-yield and high-quality cultivation model of S. sarmentosum induced by stress signal substances. Methods After foliage-spraying with different concentrations of H₂O₂ (0, 1, 10, 100 mmol/L) on S. sarmentosum, it experienced natural low temperature, and the growth index, biomass, content of various flavonoids and various in vitro antioxidant capacity were measured at harvest. Results Under low temperature conditions, spraying H₂O₂ significantly increased the maximum branch length, leaf layers, number of branches, biomass, content of various flavonoids, and in vitro antioxidant capacity. With the increase of the concentration of exogenous H₂O₂, the number of leaf layers, branches, number of new buds, biomass and peroxidase (POD) enzyme activity showed a downward trend, while enzyme activities of superoxide dismutase (SOD) and catalase (CAT) as well as malondialdehyde (MDA) content showed an upward trend. The biomass of 1 mmol/L H₂O₂ and 10 mmol/L H₂O₂ treatments increased by 209.38% and 87.50%, respectively compared with control. The content of total flavonoids, total phenolic acids, quercetin, kaempferol, and isorhamnetin were the highest when treated with 10 mmol/LH₂O₂, which was 16.67%-37.84% higher than the control, followed by treatment with 1 mmol/LH₂O₂. The S. sarmentosum treated with 1 mmol/LH₂O₂ showed have the strongest free radical scavenging ability with 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) methods, while the hydroxyl radical scavenging ability and anti-lipid peroxidation ability are the strongest when treated with 10 mmol/LH₂O₂. Conclusion H₂O₂ pretreatment under low temperature conditions is beneficial to increase the cold resistance, yield and quality of S. sarmentosum; And 1 or 10 mmol/LH₂O₂ pretreatment is suitable treatments after comprehensive evaluation.

R286.2

中央高校基本科研业务费专项资金资助（KYZZ201912）