目的 研究伸筋草Lycopodii Herba的化学成分。方法 采用多种柱色谱对伸筋草95%乙醇提取物进行分离纯化；通过波谱数据对化合物进行结构鉴定；采用SRB法对所得化合物进行细胞毒性测试。结果 从伸筋草95%乙醇提取物中分离得到7个化合物，分别鉴定为3-甲酰基-芒柄花萜醇（1 ）、lycopodine （2 ）、lycoposerramine M （3 ）、4α-hydroxy-anhydrolycodoline （4 ）、flabelliformine （5 ）、β-dihydrolycopodine （6 ）、lycodoline （7 ）。结论 化合物1 为新的serratene型三萜，命名为3-甲酰基-芒柄花萜醇。活性测试结果表明化合物1 没有明显的细胞毒活性，没有降糖活性。化合物5 、6 为首次从该植物中分离得到。

Objective To study chemical constituents of Shenjincao (Lycopodii Herba). Methods Compounds in Lycopodii Herba were isolated and purified by column chromatography on silica gel column, Sephadex LH-20 gel column, and their structures were elucidated by analysis of its NMR and HRESIMS data. The cytotoxicity of the obtained compounds was evaluated by SRB methods. Results Seven compounds were obtained from 95% ethanol aqueous extract of Lycopodii Herba and their structures were identified as 3-formyloxyonocerin (1), lycopodine (2), lycoposerramine M (3), 4α-hydroxy-anhydrolycodoline (4), flabelliformine (5),β-dihydrolycopodine (6) and lycodoline (7). Conclusion Compound 1 was a new serratene-type triterpenoid named 3-formyloxy-onocerin, which showed no cytotoxicity, but could promote the glucose absorption of L6 cells. Compound 5 and 6 are isolated from this plant for the first time.

R284.1

国家自然科学基金资助项目（81973201）；湖北省自然科学基金面上项目（2021CFB347）