目的 采用超高效液相色谱串联飞行时间质谱（UPLC-Q-TOF-MS）法快速分析石楠Photinia serrulata叶化学成分。方法 色谱柱为Waters BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm），流动相为乙腈（A）-0.1%甲酸水溶液（B），梯度洗脱，体积流量0.3 mL/min。采用peakview 1.2软件解析样品和混合对照品质谱图，得到化合物的精确相对分子质量、多级质谱数据、质谱碎片离子信息等，结合文献报道进行成分鉴定。结果 共鉴定了98个化学成分，主要包括30个黄酮类成分，24个有机酸类成分，15个苯丙素类成分，14个萜类成分和15个其他类成分，其中95个化合物是首次从该植物中发现的化学成分。结论 建立的UPLC-Q-TOF-MS法能快速、准确、较全面地鉴定石楠叶药材化学成分，为石楠叶药效物质基础研究提供依据。

Objective UPLC-Q-TOF-MS was used to quickly analyze the chemical components of Folium Photiniae. Methods The chromatographic column was waters BEH C₁₈(100 mm×2.1 mm, 1.7 μm). The mobile phase was acetonitrile (A)-0.1% formic acid water (B). The flow rate was 0.3 mL/min. To obtain the accurate molecular weight, multi-stage mass spectrum data, and mass fragment ion information, etc., peak view 1.2 software was used to analyze the mass spectra. The components were identified in combination with literature reports. Results A total of 98 chemical components were identified, mainly including 30 flavonoids, 24 organic acids, 15 phenylpropanoids, 14 terpenoids and 15 other components, of which 95 components are first identified from of Folium Photiniae. Conclusion The method established in this study can quickly, accurately and comprehensively identify the chemical constituents of Folium Photiniae, and provide the basis for the research on the material basis of its medicinal effect.

河北省中医药管理局科研计划项目(2023114);河北省自然基金资助项目(H2019423050);河北省高等学校科学技术研究项目(QN2019119)