目的 研究管花鹿药Maianthemum henryi中的甾体皂苷类成分及其细胞毒活性。方法 采用硅胶柱色谱、葡聚糖凝胶LH-20柱色谱以及半制备高效液相色谱等方法分离，根据MS、IR、NMR和GC等数据鉴定化合物的结构，MTT法测定化合物的细胞毒活性。结果 从管花鹿药乙醇提取物的正丁醇部位分离鉴定了2个甾体皂苷类化合物，分别鉴定为(23S,24S)-螺甾-5,25(27)-二烯-lβ,3β,23,24-四醇1-O-α-L-鼠李糖基-(1→2)-β-D-呋糖苷（1）和(23S,24S)-螺甾-5,25(27)-二烯- lβ,3β,21,23,24-五醇1-O-α-L-鼠李糖基-(1→2)-β-D-呋糖苷（2），化合物1和2对2种肿瘤细胞的细胞毒活性的半数抑制浓度（median inhibition concentration，IC₅₀）值均大于100 μmol/L。结论 化合物1和2为新化合物，分别命名为管花鹿药皂苷K（1）和管花鹿药皂苷L（2）；两者均未表现出细胞毒活性。

Objective To study the steroidal saponins from the roots and rhizomes of Maianthemum henryi and their cytotoxicity. Methods The chemical constituents were studied by chromatography on silica gel, Sephadex LH-20 and semi-preparative HPLC, and the structures were elucidated on the basis of spectroscopic methods, including MS, IR, NMR and GC data analysis. The cytotoxicity was studied by MTT method. Results Two new steroidal saponins were obtained from n-butanol fraction of ethanol extract of M. henryi and namely (23S,24S)-spirost-5,25(27)-diene-lβ,3β,23,24-tetraol 1-O-α-L-rhamnopyranosyl-(1→2)-β-D- furopyranoside (1) and (23S,24S)-spirost-5,25(27)-diene-lβ,3β,21,23,24-pentaol-1-O-α-L-rhamnopyranosyl-(1→2)-β-D-furopyranoside (2), and compounds 1 and 2 showed cytotoxicity against two cancer cell lines with IC₅₀ values greater than 100 μmol/L. Conclusion Compounds 1 and 2 were new compounds named henryioside K and henryioside L which possessed no cytotoxicity.

陕西省科技厅自然科学研究基础计划项目(2021JQ-742);陕西中医药大学研究生创新项目(2021CX19);陕西中医药大学学科创新团队项目(2019-YL12)