目的 建立一测多评法（quantitative analysis of multi-components by a single marker，QAMS）同时测定当归中阿魏酸、洋川芎内酯I、洋川芎内酯H、阿魏酸松柏酯、藁本内酯和丁烯基苯酞的含量。方法 采用Waters X-Select CSH色谱柱（250 mm×4.6 mm，5 μm），以0.085%磷酸-水溶液（A）-乙腈（B）为流动相，梯度洗脱，体积流量1.0 mL/min，柱温40 ℃，检测波长265 nm。结果 在线性范围内各成分线性良好（R²＞0.999）。以藁本内酯为内标，阿魏酸、洋川芎内酯I、洋川芎内酯H、阿魏酸松柏酯和丁烯基苯酞的相对校正因子分别为1.073 4、0.564 8、0.468 1、0.926 2、1.083 5；同时采用外标法测定这6个成分的含量，将2种方法进行分析比较，结果没有明显差异。结论 建立了当归药材中阿魏酸、洋川芎内酯I、洋川芎内酯H、阿魏酸松柏酯、藁本内酯和丁烯基苯酞6个成分的QAMS法，该法可用于当归的质量控制。

Objective To establish a HPLC method for the determination of ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate, ligustilide and butylidenephthalide in Danggui (Angelicae Sinensis Radix) using quantitative analysis of multi-components by a single marker (QAMS)．Methods The analysis was performed on a Waters X-Select CSH column (250 mm×4.6 mm, 5 μm), with 0.085% phosphoric acid (A) and acetonitrile (B) as mobile phase at the flow rate of 1 mL/min for gradient elution, as well as the wavelength was 265 nm and the column temperature was 40 ℃. Results All calibration curves showed good linearity (R²＞0.999) within the concentration range. Ligustilide was used as the internal reference standard, and five relative correction factors (RCF) of ferulic acid, senkyunolide I, senkyunolide H, coniferyl ferulate and butylidenephthalide to ligustilide were calculated, which were 1.073 4, 0.564 8, 0.468 1, 0.926 2 and 1.083 5, respectively. Their content in Angelicae Sinensis Radix was determined by both external standard method and QAMS method. There were no significant differences between two methods. Conclusion The method of QAMS for six components determination in Angelicae Sinensis Radix was established, which can be used for the quality control of Angelicae Sinensis Radix.

国家中药标准化项目"大黄等6种中药饮片标准化建设"(ZYBZH-Y-GS-10-B)