目的 基于ITS2和rbcL序列对来自不同地理环境的缘毛紫菀Aster souliei及狭苞紫菀A. farreri进行分子鉴定。方法 以ITS2和rbcL的特异性引物,扩增缘毛紫菀及狭苞紫菀的ITS2和rbcL的序列并测定,运用MEGA7.0软件进行多重比对分析和种间种内遗传距离分析,构建邻接法(neigbor-joining,NJ)系统发育树。结果 ITS2序列在缘毛紫菀及狭苞紫菀的变异位点较rbcL序列丰富,且种间与种内的变异位点数无交叉;ITS2序列的NJ树可将缘毛紫菀及狭苞紫菀样品进行有效区分,而rbcL序列构建的系统发育树无法使缘毛紫菀及狭苞紫菀样品得到有效的区分;ITS2及rbcL序列的遗传距离结果显示缘毛紫菀之间的遗传距离很近,而狭苞紫菀之间的遗传距离较远。结论 ITS2较rbcL序列更适合用于不同产地的缘毛紫菀及狭苞紫菀样品的鉴定;狭苞紫菀的种内差异较大。

Objective Based on the ITS2 and rbcL sequences to do molecular identification of Aster souliei andA.farreri samples from different geographical sources.Methods ITS2 and rbcL sequences ofA.souliei andA.farreri were amplified and sequenced with specific primers of ITS2 and rbcL,MEGA7.0 software was used for multiple comparison analysis and intraspecific genetic distance analysis to construct neigbor-joining (NJ) phylogenetic tree. Results The variation sites of ITS2 sequences inA.souliei andA.farreri samples were richer than those of rbcL sequences,and there was no cross between interspecific and intraspecific.The NJ tree of ITS2 sequence can effectively distinguish the samples ofA.souliei andA.farreri,while the phylogenetic tree constructed by rbcL sequence cannot effectively distinguish the samples of A.souliei andA.farreri.The genetic distance of ITS2 and rbcL sequences showed that the genetic distance amongA.souliei samples were very close,while the genetic distance among A.farreri samples were far. Conclusion ITS2 sequence is more suitable for the identification of A.souliei andA.farreri samples from different habitats than rbcL sequence.And the intraspecies ofA.farreri samples are quite different.

R286.12

国家自然科学基金项目(31870314);四川省科技厅区域创新合作项目(2020YFQ0007);西南民族大学中央高校基本科研业务专项基金项目(2021NYYXS19)