目的 研究三七Panax notoginseng花的化学成分。方法 采用70%乙醇水超声提取,醋酸乙酯与正丁醇萃取,利用D101大孔吸附树脂、硅胶、MCI gel CHP20、ODS反相柱色谱和制备液相色谱等方法进行分离和纯化,通过高分辨质谱、以及核磁共振波谱等多种光谱技术进行化合物结构解析和鉴定。结果 从三七花提取物中分离并鉴定出13个化合物,包括1个新丙二酸酰化型人参皂苷:3β,12β,20S-达玛烷型四环三萜-24-烯-3-O-[β-D-吡喃葡萄糖基(1→2)-(6-O-丙二酰基)-O-β-D-吡喃葡萄糖基]-20-O-[β-D-吡喃葡萄糖基(1→6)]-O-β-D-吡喃葡萄糖苷(1),以及12个已知化合物。其中,中性人参皂苷10个:人参皂苷Rd(2)、人参皂苷F₁(3)、人参皂苷Rb₁(4)、人参皂苷Rb₂(5)、人参皂苷Rb₃(6)、人参皂苷Rc(7)、竹节参皂苷L₅(8)、人参皂苷F₃(9)、三七皂苷FP₂(10)、三七皂苷Fa(11);黄酮类化合物2个:山柰酚-3-O-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃半乳糖苷(12)和槲皮素-3-O-β-D-吡喃葡萄糖基-(1→2)-β-D-吡喃半乳糖苷(13)。结论 化合物1为新化合物,命名为丙二酰三七花蕾皂苷Rb₁,化合物9为首次从三七植物中分离得到。

Objective To systematically investigate the chemical constituents of the flower buds ofPanax notoginseng.Methods Ultrasonic extraction by 70% aqueous ethanol,successive solvent extraction by ethyl acetate andn-butanol,normal-/reversed-phase column chromatography by D101 macroporous adsorption resin,silica gel,MCI gel CHP20,and ODS,as well as preparative HPLC,were used for isolation and purification of target compounds.A variety of spectroscopic techniques,including high-resolution MS,1D/2D NMR spectra,etc.were used for the structural identification of the isolated compounds.Results Thirteen compounds were isolated and identified,including a new malonylginsenoside:3β,12β,20S-dammarane-24-ene-3-O-[β-D-glucopyranoside (1→2)-(6-O-malonyl)-O-β-D-glucopyranosyl]-20-O-[β-D-glucopyranosyl (1→6)]-O-β-D-glucopyranoside (1),together with 12 known compounds,including 10 neutral ginsenosides:ginsenoside Rd (2),ginsenoside F₁(3),ginsenoside Rb₁(4),ginsenoside Rb₂(5),ginsenoside Rb₃(6),ginsenoside Rc (7),chikusetsusaponin L₅(8),ginsenoside F₃(9),notoginsenoside FP₂(10),and notoginsenoside Fa (11),as well as two flavonoids:kaempferol-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-galactopyranoside (12),and quercetin-3-O-β-D-glucopyranosyl-(1→2)-O-β-D-galactopyranoside (13).Conclusion Compound 1 named as malonylfloralnoto-ginsenoside Rb₁ is a new structure,and compound 9 is isolated from this plant for the first time.

R284.1

国家自然科学基金面上项目(81872996)