[关键词]
[摘要]
目的 研究NaCl胁迫下掌叶大黄Rheum palmatum、唐古特大黄R.tanguticum和药用大黄R.officinale种子萌发及幼苗生长特性及赤霉素(GA3)引发对盐胁迫下种子萌发及幼苗发育的影响。方法 设置0、100、150、200、250 mmol/L NaCl胁迫,200、250 mg/L GA3拌种或浸种实验,双层滤纸培养法分析盐胁迫下3种大黄种子萌发及幼苗生长指标。结果 3种大黄种子发芽率随NaCl浓度增加直线下降、盐相对伤害率直线上升(P<0.05),子叶长、胚轴长、根长和苗长等受到强烈抑制(P<0.05)。200 mmol/L NaCl下GA3拌种或浸种能显著提高掌叶大黄种子发芽率(P<0.05),250 mmol/LNaCl下仅GA3拌种有作用(P<0.05);200 mmol/L NaCl下2个质量浓度GA3拌种、低质量浓度浸种提高唐古特大黄种子发芽率(P<0.05),250 mmol/L NaCl下GA3无促进作用(P>0.05);GA3拌种和高质量浓度GA3浸种能提高150 mmol/L NaCl下药用大黄种子发芽率(P<0.05),100 mmol/L NaCl下GA3无促进作用(P>0.05)。GA3浸种能促进盐胁迫下掌叶大黄、唐古特大黄和药用大黄幼苗根和苗的生长(P<0.05)。结论 NaCl胁迫下3种大黄种子萌发及幼苗生长均受到抑制,3种大黄种子和幼苗耐盐性有差异,药用大黄耐盐性最低,GA3拌种或浸种引发能不同程度缓解高盐胁迫下大黄种子萌发和幼苗发育。
[Key word]
[Abstract]
Objective To reveal the characteristics of seed germination and seedling growth of Rheum palmatum, R. tanguticum and R. officinale and the effects of gibberellin (GA3) priming on seed germination and seedling development under high salinity stress. Methods The double-layer filter paper culture method was used for the analyses of seed germination and seedling growth of three rhubarbs under various treatments including 0, 100, 150, 200, 250 mmol/L NaCl stresses, and seed dressing or seed soaking with 200 and 250 mg/L GA3, respectively. Results Seed germination rates of three rhubarbs decreased and the relative injury rate of salinity increased with the increasement of NaCl concentration (P < 0.05). The cotyledon, hypocotyl, root and seedlings length were all strongly inhibited with the increasement of NaCl concentration (P < 0.05). Seed dressing or soaking with GA3 could significantly improve the germination rate of R. palmatum under 200 mmol/L NaCl (P < 0.05), and under 250 mmol/L NaCl only seed dressing had effect (P < 0.05). Seed dressing with two concentrations of GA3 and seed soaking at low concentration of GA3 improved the germination rate of R. tanguticum under 200 mmol/L NaCl (P < 0.05), and under 250 mmol/L NaCl GA3 had no promoting effect (P > 0.05). Seed dressing with GA3 and seed soaking with high concentration GA3 enhanced the seed germination rate of R. officinale under 150 mmol/L NaCl (P < 0.05), and under 100 mmol/L NaCl GA3 had no promoting effect (P > 0.05). Seed soaking with GA3 could promote the growth of root and seedling for R. palmatum, R. tanguticum and R. officinale under the included salinity stresses (P < 0.05). Conclusion Seed germination and seedling growth of the three rhubarbs under the included NaCl stressesall showed repressed. The three species displayed different tolerance at the seed and seedlings stages against salinity stress, and R. officinale was the least. Either Seed dressing or soaking with GA3 priming could alleviate the seed germination and seedling development of rhubarbs under high salinity stresses.
[中图分类号]
R286.12
[基金项目]
国家自然科学基金资助项目(81973430);国家自然科学基金资助项目(82104334);陕西省重点产业创新链(2021ZDLSF04-01);陕西省中医药管理局重点专项(2021-QYZL-02);陕西省教育厅重点项目(20JY013);省部共建特色秦药资源研究开发国家重点实验室(培育)开放课题(QY202101);陕西中医药大学科创新团队项目(2019-QN01);中央本级重大增减支项目(2060302)