目的 针对白芍Paeonia lactiflora在传统加工炮制过程中成分流失、生产工序重复等问题，利用响应面法优选白芍产地加工与炮制生产一体化（简称“一体化”）工艺。方法 采用Box-Behnken响应面试验设计方法考察白芍一体化工艺中的煮制时间（X₁）、鲜药材干燥时间（X₂）、鲜药材干燥温度（X₃）3个因素，以4种指标性成分（儿茶素、芍药内酯苷、芍药苷、1,2,3,4,6-O-五没食子酰基葡萄糖）的总评归一值（OD值）作为评价指标，进行一体化工艺优化研究。结果 白芍一体化最佳工艺条件为煮制时间14.11 min，鲜药材干燥时间4.51 h，鲜药材干燥温度52.53℃。结论 Box-Behnken响应面法优选白芍一体化工艺切实可行，具有较强的实际意义，为白芍饮片一体化加工生产提供了科学依据。

Objective In order to solve the problems of component loss and repeated production in the traditional processing process of Paeoniae Radix Alba (PRA), the integrated technology of primary processing for PRA was optimized by response surface methodology. Methods The Box-Behnken response surface design methodology was used to investigate the three factors of boiling time, fresh medicinal material drying time and temperature in the integrated process. The integrated process optimization was studied by using the overall desirability value (OD value) of catechin, albiflorin, paeoniflorin, and 1,2,3,4,6-O-pentagalloyl glucose in PRA as evaluation indexes. Results The results showed that the best integrated technological conditions were as follows:boiling time 14.11 min, drying time 4.51 h and drying temperature 52.53℃. Conclusion The study showed that the response surface methodology is simple and easy to optimize the integrated primary processing for PRA and has good accuracy, which provides a scientific reference for the integrated processing and production of PRA.

R283.6

国家重点研发计划“中医药现代化研究”专项（2019YFC1711500）