目的 分析过路黄Lysimachia christinae MYB转录因子家族相关成员的结构和功能，挖掘与黄酮类物质生物合成相关的基因信息。方法 基于过路黄全长转录组数据库，利用ProtParam、GenStript、MEME、WebLogo、SOPMA、Swiss-Model等在线网站分析过路黄MYB转录因子家族蛋白的理化性质、亚细胞定位、保守基序和结构域、蛋白高级结构等。利用MEGA软件构建系统发育树，分析过路黄MYB转录因子家族（ LcMYB）与拟南芥Arabidopsis thaliana MYB蛋白的系统发育关系。结果 挖掘到51个LcMYB基因，预测51条具有MYB转录因子保守结构域的蛋白序列；根据结构域可分为R1-LcMYB、R2R3-LcMYB和R3-LcMYB 3个亚类，且所有LcMYB基序中都含有3个保守的色氨酸；过路黄MYB家族蛋白均为亲水性蛋白，热稳定性较高且富含碱性氨基酸，大部分蛋白以无规则卷曲为主，其中R2R3-LcMYB39、R2R3-LcMYB47、R2R3-LcMYB48与R2R3-LcMYB50蛋白是以α-螺旋为主；与拟南芥MYB转录因子家族共同构建的进化树发现，过路黄MYB家族在进化上包括3个大类。结论 在过路黄物种中获得了51个MYB转录因子家族成员的基因的结构和功能信息，其中有2个成员与黄酮类物质的生物合成有关，该研究结果为后续克隆利用黄酮类物质生物合成相关基因提供了分子基础。

Objective To analyze the structure and function of the members of MYB transcription factor family in Lysimachia christinae, and excavate some information of the genes related to flavonoid biosynthesis. Methods Based on transcriptome database of L. christinae, the physicochemical properties, subcellular localization, conserved motifs and domains and protein structures of the LcMYB were analyzed by online websites, including ProtParam, GenStript, MEME, WebLogo, SOPMA, Swiss-Model, etc. The MYB phylogenetic tree was constructed by MEGA to analyze the phylogenetic relationship between L. christinae and Arabidopsis thaliana. Results A total of 51 LcMYB genes were identified, 51 MYB protein sequences with conserved domain were predicted. LcMYB can be divided into three classes (R1-LcMYB, R2R3-LcMYB and R3-LcMYB) according to the conserved domain, and all the LcMYB motifs contained three conserved tryptophan. All the MYB proteins of L. christinae were hydrophilic and rich in basic amino acids, with high thermal stability. Most of the proteins were mainly in random curl, among them, R2R3-LCMYB39, R2R3-LCMYB47, R2R3-LCMYB48 and R2R3-LCMYB50 were mainly in α-helix. MYB family of L. christinae could be divided into three groups on the basis of evolutionary tree with the MYB family of A. thaliana. Conclusion The structure and function information of 51 genes associated with MYB transcription factor family members was obtained in L. christinae, thereinto, two genes were involved in the biosynthesis of flavonoids, which provided molecular basis for the subsequent cloning of genes related to the biosynthesis of flavonoids.

R282.12

西华师范大学校基本科研业务费（19B033）；青海民族大学校级理工科项目（2019XJY02）；绵阳师范学院科研启动项目（QD2019A13）