目的 研究太白银莲花Anemone taipaiensis地上部分化学成分，并初步评估其肿瘤细胞增殖抑制活性。方法 采用正相硅胶、Sephadex LH-20凝胶、ODS反相柱色谱以及半制备高效液相色谱等手段进行分离纯化；根据波谱数据（1D-NMR、2D-NMR及MS）和理化性质鉴定化合物结构；采用MTT法评估化合物对2种人源肿瘤细胞（人急性早幼粒白血病HL-60细胞和人肝癌HepG2细胞）的增殖抑制活性。结果 从太白银莲花地上部分70%乙醇提取物的正丁醇萃取部位分离得到7个齐墩果烷型三萜皂苷类化合物，分别鉴定为常春藤皂苷元-3β-O-β-D-吡喃葡萄糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖(1→4)-β-D-吡喃葡萄糖苷（1）、3β-O-β-D-吡喃木糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖-齐墩果酸-28-O-β-D-吡喃葡萄糖酯苷（2）、cernusoide C（3）、medicago-saponin P₁（4）、常春藤皂苷元-3β-O-β-D-吡喃木糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-[β-D-吡喃葡萄糖-(1→4)]-α-L-吡喃阿拉伯糖苷（5）、hederacholichiside F（6）和3β-O-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖-齐墩果酸-28-O-α-L-吡喃鼠李糖-(1→4)-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖-(1→4)-α-L-吡喃鼠李糖-(1→4)-β-D-吡喃葡萄糖-(1→6)-β-D-吡喃葡萄糖酯苷（7）；化合物1、2和5显示出不同程度的肿瘤细胞增殖抑制活性。结论 化合物1为新化合物，命名为太白银莲花皂苷C；化合物4为首次从银莲花属植物中分离得到；化合物5对HL-60细胞和HepG2细胞有显著增殖抑制活性，半数抑制浓度（median inhibition concentration，IC₅₀）值分别为5.79和3.42 μmol/L。

Objective To study the chemical constituents from the aerial parts of Anemone taipaiensis and their preliminary antiproliferatory activity in tumor cells. Methods The n-butanol extract of 70% ethanol extract from the aerial parts of this plant was separated by normal silica gel, Sephadex LH-20 gel, reversed phase ODS column chromatography and semi-preparative high performance liquid chromatography. The structures of obtained compounds were identified by spectral analysis (1D-NMR, 2D-NMR and MS) and physicochemical properties. The antiproliferatory activity against two human tumor cells (HL-60 and HepG2) was evaluated by MTT assay. Results Seven oleanane-type triterpenoid saponins were obtained and identified as hederagenin- 3β-O-β-D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranosyl-(1→4)-β-D-glucopyranoside (1), 3b-O-b-D- xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-a-L-arabinopyranosyl-oleanolic acid-28-O-β-D-glucopyranosyl ester (2), cernusoide C (3), medicago-saponin P₁ (4), hederagenin-3b-O-b-D-xylopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)-[β-D- glucopyranosyl-(1→4)]-a-L-arabinopyranoside (5), hederacholichiside F (6) and 3b-O-α-L-rhamnopyranosyl-(1→2)-a-L- arabinopyranosyl-oleanolic acid-28-O-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl-(1→4)-α-L- rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl ester (7). Compounds 1, 2 and 5 displayed antiproliferation activities on tumor cells. Conclusion Compound 1 is a new compound named taipaienoside C. Compound 4 is isolated form the genus Anemone for the first time. Compound 5 displayed significant antiproliferation activity against HL-60 cells and HepG2 cells with IC₅₀ values of 5.79 and 3.42 μmol/L, respectively.

R284.1

国家自然科学基金资助项目（81274029）；北京市属医院科研培育计划（PZ2021025）；北京大学肿瘤医院科研基金资助项目（2021-26）