目的 通过比较性状、浸出物、生物碱和黄酮成分的含量、指纹图谱等内容来系统研究基原为酸橙Citrus aurantium和甜橙C.sinensis的枳实药材质量差异，以期建立一个多元的枳实药材质量控制体系。方法 按照《中国药典》2020年版枳实项下对酸橙和甜橙枳实药材进行质量评价和比较，通过HPLC法对枳实进行定性及定量分析，分别建立不同基原枳实药材的指纹图谱并对其9个主要的黄酮成分进行含量测定。结果 不同基原的枳实药材浸出物含量差异明显，而辛弗林含量没有显著性差异；甜橙枳实中不含新橙皮苷，而酸橙枳实中该成分含量最高；除桔皮素外，2个基原枳实的其他黄酮成分含量均存在显著性差异（P＜0.05）；通过指纹图谱的模式识别，2种枳实分类情况与其产地分布较为吻合；通过正交偏最小二乘判别分析筛选出了柚皮苷、橙皮苷、新橙皮苷这3个差异性成分。结论 不同基原枳实药材在浸出物含量、主要黄酮类成分的种类及含量等方面均存在一定的差异性，对其进行的质量差异研究及指纹图谱的比较，可以为枳实临床用药选择、新药开发、药材质量控制等方面提供依据。

Objective The quality differences of two origins of Zhishi (Aurantii Fructus Immaturus) were studied by comparing their characters, extractives, synephrine, fingerprint and flavonoid contents. Methods The quality of Aurantii Fructus Immaturus was evaluated and compared with that of Aurantii Fructus Immaturus under the items of character, extract and content determination of Aurantii Fructus Immaturus in Chinese Pharmacopoeia (2020 Edition). Then, the qualitative and quantitative analysis of Aurantii Fructus Immaturus was carried out by HPLC, and the fingerprints of different Aurantii Fructus Immaturus were established and the nine main flavonoids were determined. Results There was significant difference in the content of extracts from different varieties of Aurantii Fructus Immaturus, but no significant difference in the content of synephrine. There was no new hesperidin in sweet orange immature orange, but the content of this component was the highest in Citrus Aurantii. There was a significant difference in the content of flavonoids in the two varieties except for tangerine peel (P<0.05). Through the pattern recognition of fingerprint, the classification of the two origins of Aurantii Fructus Immaturus was consistent with the distribution of its origin. Three different components, naringin, hesperidin and neohesperidin were selected by OPLS-DA analysis. Conclusion There are some differences in the extract content, types and contents of the main components of different origins of Aurantii Fructus Immaturus. The quality difference research and fingerprint comparison in this experiment can provide the basis for clinical drug selection, new drug discovery and quality control of Aurantii Fructus Immaturus.

R286.2

江苏高校优势学科建设工程项目(PAPD)