目的 研究窄叶鲜卑花Sibiraea angustata地上部分的化学成分及其体外降血脂活性。方法 采用硅胶柱色谱、ODS反相柱色谱、半制备液相色谱等方法进行分离纯化，利用NMR、MS等技术鉴定化合物结构，再通过建立人肝癌HepG2细胞高脂模型测试各化合物调脂活性。结果 从窄叶鲜卑花的95%乙醇提取物中分离得到了21个化合物，分别鉴定为藜芦酸甲酯（1）、阿魏酸甲酯（2）、3-羟基-4-甲氧基肉桂酸甲酯（3）、3,4,5-三甲氧基肉桂醇（4）、对羟基桂皮酸甲酯-4-O-β-D-吡喃葡萄糖苷（5）、藜芦酸葡萄糖苷（6）、aglycone（7）、aruncide A（8）、3-[(2E)-4-hydroxyl-4-methyl-2-pentenyl)]furan-5H-2-one（9）、吐叶醇（10）、蚱蜢酮（11）、3,7-dimethyl-3(Z),6-octadien-5-one-1-O-β-D-glucoside（12）、(2R,3S,4S)-4-(4-hydroxy-3-methoxybenzyl)-2-(5-hydroxy-3-methoxyphenyl)-3-(hydroxymethyl)-tetrahydrofuran-3-ol（13）、1,2-bis (4-hydroxy-3-methoxyphenyl)-1,3-propanediol（14）、salicifoneoliganol（15）、herpetriol（16）、达玛烯二醇-II（17）、山楂酸甲酯（18）、麦珠子酸（19）、阿魏酸二十四烷酯（20）、对羟基反式肉桂酸二十四烷基酯（21）。结论 化合物1～8、10、11、13～21为首次从鲜卑花属植物中分离得到，化合物7、8、17、18在体外实验中显示出降脂活性。

Objective To study the chemical composition from the aerial part of Sibiraea angustata and their activity in vitro. Methods Silica gel column chromatography, ODS reversed-phase column chromatography, semi-preparative liquid chromatography were used for separation and purification. NMR, MS and other techniques were used to identify the structure of the isolated compounds, and the lipid-lowering activities of each compound were tested by establishing HepG2 cell hyperlipid model. Results Twenty-one compounds were separated from 95% ethanol extract of Sibiraea angustata, which were identified as methyl veratrate (1), (E)-3,4-dimethoxycinnamic acid methyl ester (2), (E)-methyl 3-(3-hydroxy-4-methoxyphenyl) acrylate (3), 3,4,5-trimethoxycinnamyl alcohol (4), linocinnamarin (5), tecomin (6), aglycone (7), aruncide A (8), 3-[(2E)-4-hydroxyl-4-methyl-2-pentenyl)]furan-5H-2-one (9), vomifoliol (10), grasshopper ketone (11), 3,7-dimethyl-3(Z),6-octadien-5-one-1-O-β- D-glucoside (12), (2R,3S,4S)-4-(4-hydroxy-3-methoxybenzyl)-2-(5-hydroxy-3-methoxyphenyl)-3-(hydroxymethyl)-tetrahydrofuran-3-ol (13), 1,2-bis(4-hydroxy-3-methoxyphenyl)-1,3-propanediol (14), salicifoneoliganol (15), herpetriol (16), dammarenediol- II (17), methyl maslinat (18), alphitolic acid (19), tetracosyl ferulate (20), n-tetracosyl trans-p-coumarate (21). Conclusion 19 compounds (1-8, 10, 11, 13-21) are isolated from Sibiraea genus for the first time; Compounds 7, 8, 17 and 18 showed lipid-lowering activity in vitro.

R284.1

国家自然科学基金资助项目(81760708);江西省主要学科学术和技术带头人资助计划项目(20162BCB22016);江西省教育厅重点项目(GJJ160809)