目的 研究华中五味子Schisandra sphenanthera根皮的化学成分及其细胞毒活性。方法 采用硅胶柱色谱、sephadex LH-20葡聚糖凝胶柱色谱和半制备液相色谱进行分离纯化，结合NMR、MS和ECD等数据进行化合物结构鉴定，采用MTT法测定化合物的细胞毒活性。结果 从华中五味子根皮中分离得到11个化合物，分别鉴定为(7R,10S,11S)-2,11,12-三羟基菖蒲烯（1）、五味子醇丁（2）、12-demethylwuweilignan I（3）、戈米辛M1（4）、异戈米辛O（5）、schisphenlignan C（6）、marlignan F（7）、wilsonilignan A（8）、sphenlignan A（9）、schinegllignan A（10）、schinegllignan B（11），化合物2～5和7对人肺癌A549细胞、人结直肠癌HCT116细胞和SW620细胞具有细胞毒活性，半数抑制浓度（median inhibition concentration，IC₅₀）值为12.1～38.7 μmol/L。结论 化合物1为新的倍半萜类化合物，命名为华中五味子倍半萜A；化合物2为新天然产物，化合物3～5、7、8、10～11首次从华中五味子中分离得到，化合物2～5和7具有细胞毒活性。

Objective To study the chemical constituents from the root bark of Schisandra sphenanthera and their cytotoxic activities. Methods The compounds were isolated and purified by silica, sephadexLH-20 and semi preparative-HPLC and the chemical structures were identified by NMR, MS and ECD data analysis. Results Eleven compounds were isolated and deduced as:(7R,10S,11S)-2,11,12-trihydroxycalamenene (1), schisandrol D (2), 12-demethylwuweilignan I (3), gomisin M1 (4), isogomisin O (5), schisphenlignan C (6), marlignan F (7), wilsonilignan A (8), sphenlignan A (9), schinegllignan A (10), schinegllignan B (11). Compounds 2-5 and 7 showed cytotoxic activity against A549, HCT116 and SW620 cell lines with IC₅₀ values ranging from 12.1 to 38.7 μmol/L. Conclusion Compound 1 is a new sesquiterpene, named as schisphensesquiterpene A, and compound 2 is a new natural product. Compounds 3-5, 7, 8, 10-11 were isolated from the plant for the first time, and compounds 2-5 and 7 exhibited cytotoxic activities.

R284.1

国家自然科学基金项目(82174111);国家自然科学基金项目(82104368);陕西中医药大学学科创新团队项目(2019-YL12)