目的 优选羟丙基-β-环糊精（2-hydroxypropyl-β-cyclodextrin，HPCD）包合香果健消片（Xiangguo Jianxiao Pian，XJP）挥发油最佳工艺。方法 在单因素实验基础上，以包合率、收率、含油率、每克包合物中1,8-桉叶素的含量为指标，HPCD与无水乙醇的质量体积比、包合温度、包合时间为影响因素，采用信息熵权法确定各指标权重系数，计算综合评分，建立正交试验优选最佳包合工艺。采用薄层色谱、紫外吸收光谱、傅里叶红外光谱、差示扫描量热法、扫描电镜、X射线衍射、GC-MS等方法对包合物进行表征，并考察其稳定性影响因素。结果 最佳包合工艺为HPCD与无水乙醇的质量体积比为12∶20（g/mL），包合温度55℃，包合时间2 h，经验证制得的包合物平均包合率为88.15%、收率为96.11%、含油率为7.65%、包合物中1,8-桉叶素的含量为20.28 mg/g。挥发油成功进入HPCD空腔中，包合前后挥发油成分基本未发生变化，包合物稳定性较好。结论 优选的挥发油包合工艺稳定可行。

Objective To optimize the best process of 2-hydroxypropyl-β-cyclodextrin (HPCD) inclusion Xiangguo Jianxiao Pian (XJP, 香果健消片) volatile oil. Methods On the basis of single factor experiments, taking inclusion rate, yield, oil content, and the content of eucalyptol per gram of inclusion compound as indicators, and the ratio of HPCD to absolute ethanol, inclusion temperature, and inclusion time were influencing factors. The information entropy method was used to determine the weight coefficient of each index, then calculate the comprehensive score, the inclusion process conditions were optimized by orthogonal experiment. The inclusion complex was verified by thin layer chromatography, ultraviolet absorption spectroscopy, Fourier infrared spectroscopy, differential scanning calorimetry, scanning electron microscopy, X-ray diffraction, GC-MS, and investigated the factors affecting stability of the inclusion compound. Results The best inclusion process was that the ratio of HPCD to absolute ethanol was 12:20(g/mL), the inclusion temperature was 55℃, and the inclusion time was 2 h. The average inclusion rate of the obtained inclusion compound was 88.15%, the yield was 96.11%, the oil content was 7.65%, and the content of eucalyptol per gram of the inclusion compound was 20.28 mg. The volatile oil successfully entered the HPCD cavity, and the composition of the volatile oil remained basically unchanged before and after the inclusion, and the inclusion compound had good stability. Conclusion The preferred volatile oil inclusion process is stable and feasible.

R283.6

国家自然科学基金资助项目(82060723);国家自然科学基金资助项目(82174065)