目的 比较不同产地和生长年限栽培滇重楼Paris polyphylla var.yunnanensis生物量和性状差异，并对植株生物量积累和生长发育规律进行异速生长分析。方法 使用Pearson相关分析研究滇重楼根茎生物量与采集地经纬度、气候因子间的相关性；单因素方差分析（One-Way ANOVA）与独立样本T检验用于样品间植物性状的差异显著性比较；利用标准化主轴回归分析（standardized major axis regression）对滇重楼的生物量积累和生长发育规律进行异速生长研究。结果 研究表明12个不同产地的5年生滇重楼根茎生物量平均值在1.19～19.73 g，不同产地之间具有显著差异（P＜0.05），但该差异与产地经纬度、年均温度、年均降水量之间不存在显著相关性（P＞0.05）。滇重楼植株的根茎生物量均随生长年限增加而增加，种植5年后根茎生物量一直呈现快速积累趋势。相同生长年限植株中，云南省元阳县样品根茎生物量显著高于姚安县样品（P＜0.05），并且数值具有更高的变化范围。滇重楼植株的总生物量、株高、叶生物量、叶长、叶宽、叶面积均随生长年限增加而增加，比叶面积均随生长年限增加而减小。云南省元阳县滇重楼样品根茎生物量占总生物量的比例随生长年限增加而增加，云南省姚安县样品根茎生物量比例不随生长年限变化而变化。滇重楼植株总生物量和根茎生物量、株高、叶生物量、比叶面积之间均存在异速生长关系。与云南省姚安县滇重楼样品相比，云南省元阳县滇重楼样品根茎生物量积累速率高、株高较矮、叶生物量积累速率较低、比叶面积较大。结论 培育高产滇重楼，应考虑选择株高较矮、叶片较大而薄的地理种源；人工栽培滇重楼时需要注意合理控制光照等因素，从而提高滇重楼药用部位产量。

Objective The differences of biomass and traits of cultivated Paris polyphyllavar.yunnanensisin different geographic origins and growth years were compared. And allometry analysis was carried out on the law of biomass accumulation, growth and development in plants. Methods Using Peareson correlation analysis to study the relevance between the rhizome biomass of P. polyphyllavar.yunnanensis and latitude, longitude, climate factors of collection site. One-way analysis of variance (One-Way ANOVA) and independent sample t-test were used to compare the significant differences in plant traits between samples. The allometry of the biomass accumulation, growth and development of P. polyphylla var. yunnanensis was studied by standardized major axis regression. Results Our research showed that the mean value of the rhizome biomass of the 5-year-old plants was 1.19-19.73 g, and there were significant differences among 12 origins (P< 0.05), which did not significantly correlate with the longitude, latitude, mean annual temperature, and mean annual precipitation of the origins (P>0.05). The rhizome biomass of P. polyphyllavar.yunnanensis increased with the growing age, and which even increased more rapidly after growing for five years. Among the same growing age plants, the samples of rhizome biomass from Yuanyang was significant higher (and coefficient of variation of the rhizome biomass) than the ones from Yao'an in Yunan Province (P< 0.05). The total biomass, plant height, leaf biomass, leaf length, leaf width, and leaf area increased with growing age, whereas the specific leaf area (SLA) decreased with the growing age. The rhizome biomass/total biomass ratio increased with growing age in the samples from Yuanyang, while it didn't change with growing age in the samples from Yao'an. Allometry relationships existed in rhizome biomass vs. total biomass, plant height vs. total biomass, leaf biomass vs. total biomass, and SLA vs. total biomass, respectively. Compared the samples from Yao'an, the samples from Yuanyang had higher accumulation rate for rhizome biomass, shorter height, lower accumulation rate for leaf biomass, and higher SLA. Conclusion The results indicated that the geographical provenances with shorter height, large and thin leaves could be selected for maximizing the P. polyphyllavar.yunnanensis yield. Furthermore, factors such as light should be considered when cultivatingP. polyphyllavar.yunnanensis in order to increase the yield of the medicinal part of it.

R286.2

国家自然科学基金项目(81460584);云南省科技计划项目(2017RA001)