目的 采用超高效液相色谱串联四极杆飞行时间质谱联用技术（UPLC-Q-TOF-MS）对人参配方颗粒标准汤剂化学成分进行快速表征，阐明人参配方颗粒的化学物质基础，并建立指纹图谱方法对人参配方颗粒进行质量控制。方法 采用ACQUITY UPLC HSS T3色谱柱（100 mm×2.1 mm，1.8 μm），以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱，在正、负离子模式下分别采集人参配方颗粒标准汤剂的质谱数据，通过MS DIAL软件辅助解析、数据库检索和对照品比对等方法快速分析人参配方颗粒标准汤剂化学成分。并采用ACQUITY UPLC HSS T3色谱柱（150 mm×2.1 mm，1.8 μm），乙腈-0.01%磷酸水溶液为流动相，建立人参配方颗粒指纹图谱方法。结果 从人参配方颗粒标准汤剂中共鉴定出57个化合物，包括皂苷25个、氨基酸及其衍生物12个、碱基及核苷5个、其他类成分15个。建立的人参配方颗粒指纹图谱有8个共有峰，分别为人参皂苷Rg₁、Re、Rf、Rc、Ro、Rb₁、Rb₂、Rd。指纹图谱方法精密度、稳定性、重复性良好，不同厂家不同批次人参配方颗粒的成分基本相同，仅含量上存在一定差异。结论 该研究基本阐明了人参配方颗粒的化学物质基础，建立的指纹图谱方法为人参配方颗粒的质量控制提供了方法参考。

Objective Ultra performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was adopted to rapidly characterize the chemical constituents from standard decoction of Ginseng Dispensing Granules, elucidate the chemical basis of Ginseng Dispensing Granules, and establish an HPLC fingerprint for their quality control. Methods An ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) column was used for the gradient elution with acetonitrile (A)-0.1% formic acid (B) as the mobile phases, and the data was collected in positive and negative modes. The chemical constituents of Ginseng Dispensing Granules were analyzed and identified by MS DIAL software, comparing with reference standards and searching database. The HPLC fingerprint was established on an ACQUITY UPLC HSS T3 column (2.1 mm×150 mm, 1.8 μm) with acetonitrile −0.01% phosphoric acid as the mobile phases. Results A total of 57 compounds, including 25 saponins, 12 amino acids and their derivatives, five bases and nucleosides, together with 15 other compounds, were identified in the standard decoction of Ginseng Dispensing Granules. The HPLC fingerprint of Ginseng Dispensing Granules was established with eight common peaks, respectively ginsenoside Rg₁, ginsenoside Re, ginsenoside Rf, ginsenoside Rc, ginsenoside Ro, ginsenoside Rb₁, ginsenoside Rb₂, and ginsenosede Rd. And the precision, stability and reproducibility of the HPLC fingerprint were acceptable. The components in different batches of Ginseng Dispensing Granules from different manufacturers were basically similar, and only slight differences existed in contents. Conclusion The results of this study clarified the chemical substance basis of Ginseng Dispensing Granules, and the established HPLC fingerprint provided a method reference for the quality control of Ginseng Dispensing Granules.

R284.1

国家自然科学基金重点项目(82130111);国家自然科学基金青年科学基金资助项目(82003938)