目的 通过对杜仲LBD基因家族进行全面鉴定,以期为EuLBDs基因功能深入研究提供依据。方法 以杜仲基因组数据库为基础,利用生物信息学方法,对杜仲LBD基因家族进行全面鉴定和表达模式分析。结果 从杜仲基因组中共鉴定到19个EuLBDs,分为Class I与Class II 2大类,进一步划分为6个亚家族(Ia、Ib、Ic、Id与IIa、IIb)。理化性质分析显示:EuLBDs编码152~293个氨基酸,理论等电点分布于4.62~9.91,相对分子质量区域为17 420~31 820,均为亲水性蛋白,以α-螺旋和不规则卷曲为主,亚细胞定位于细胞核中。EuLBD基因家族含有类锌指、亮氨酸拉链和甘氨酸-丙氨酸-丝氨酸(GAS)保守结构域。表达模式分析显示,EuLBDs参与杜仲叶片发育,随着叶片发育,水平逐渐降低,EuLBDs基因表达不受叶片胶含量的影响。结论 从杜仲基因组水平对LBD基因家族进行了全面鉴定和生物信息学分析,为进一步研究杜仲LBDs基因功能奠定基础。

Objective In order to provide a basis for further research on the function of EuLBDs gene, the LBD gene family of Eucommia ulmoides was comprehensively identified in this study. Methods Based on E. ulmoides genome database, this study used bioinformatics methods to identify and analyze E. ulmoides LBD gene family. Results A total of 19 EuLBDs were identified from E. ulmoides genome, which were divided into Class I and Class II groups and further divided into six subfamilies (Ia, Ib, Ic, Id and IIa, IIb). EuLBDs encodes 152—293 amino acids, and the theoretical isoelectric points distribution were 4.62—9.91, molecular weight between 17 420—31 820 by physicochemical analysis, all of EuLBD proteins were hydrophilic proteins. α-Helix and irregular curl were the main structure of the secondary structure in the EuLBD gene family which was mainly expressed in nucleus. EuLBD gene family had sinusoidal structure, the leucine zipper and the glycine-alanine-serine (GAS) structure domain. Expression pattern analysis showed that EuLBDs involved in E. ulmoides leaves development, and the transcription level of EuLBDs gradually decreased with leaf development, which were not affected by the leaf gum content. Conclusion In this study, the members of the LBD family were comprehensively identified and bioinformatics analysis from the genomic level of E. ulmoides,which laid a foundation for further functional research of EuLBD genes.

R286.12

河南省高等学校重点科研项目（22A360005）；国家林业和草原局/北京市共建竹藤科学与技术重点实验室开放基金(ICBR-2020-05)；国际竹藤中心安徽太平试验中心开放课题基金(1632021006-4)；河南中医药大学博士科研基金资助项目（RSBSJJ2019-04）