目的 探讨人参固本口服液对小鼠股骨骨折术后认知功能的影响及作用机制。方法 60只C57BL/6小鼠随机分为对照组、人参固本口服液(0.50 mL/只)组、手术组和手术+人参固本口服液低、中、高剂量(0.05、0.10、0.50 mL/只)组。小鼠麻醉后制备骨折模型,各给药组ig相应药物;术后第3天,采用Morris水迷宫实验记录小鼠逃避潜伏期,Y迷宫检测小鼠学习记忆次数,旷场实验检测其情绪行为;行为学完成后,小鼠处死取海马组织,采用ELISA法检测小鼠海马区肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素-6(interleukin-6,IL-6)水平;采用qRT-PCR法测定海马区B淋巴细胞瘤2(B-cell lymphoma 2,Bcl-2)和Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax)mRNA表达;采用TUNEL法检测海马神经元凋亡情况。结果 与手术组比较,手术+人参固本口服液高剂量组小鼠术后第3天的平均逃避潜伏期显著缩短(P<0.05),学习记忆次数显著减少(P<0.05),穿越次数显著增加(P<0.05);血清中TNF-α和IL-6水平明显降低(P<0.05);海马区Bax mRNA表达水平显著降低(P<0.05),Bcl-2 mRNA表达水平显著升高(P<0.05);海马组织神经细胞凋亡数显著减少(P<0.05)。结论 人参固本口服液能够通过抑制炎症反应,降低海马神经元凋亡,从而改善小鼠股骨骨折术后认知功能障碍。

Objective To investigate the effect and mechanism of Ginseng Guben Oral Liquid (人参固本口服液, RSGB) on cognitive function of mice after femoral fracture surgery. Methods Sixty C57BL/6 mice were randomly divided into control group, RSGB (0.50 mL/mice) group, surgery group and surgery + RSGB low-, medium- and high-dose (0.05, 0.10, 0.50 mL/mice) groups. Mice were anesthetized to prepare a fracture model, and each administration group was given corresponding drugs. On 3rd day after operation, escape latency of mice was recorded by Morris water maze test, learning and memory times of mice were detected by Y-maze, and emotional behavior of mice was detected by open field test; After behavioral study, mice were sacrificed and hippocampal tissue was taken. ELISA method was used to detect tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in hippocampus of mice; qRT-PCR was used to determine the mRNA expressions of B-cell lymphoma 2 (Bcl-2) and Bcl-2 associated X protein (Bax) in hippocampus; TUNEL method was used to detect the apoptosis of hippocampal neurons. Results Compared with operation group, average escape latency of mice in operation + RSGB high-dose group was significantly shortened on 3rd day after operation (P < 0.05), learning and memory times were significantly reduced (P < 0.05), and crossing times were significantly increased (P < 0.05); TNF-α and IL-6 levels in serum were significantly decreased (P < 0.05); Bax mRNA expression in hippocampus was significantly decreased (P < 0.05), and Bcl-2 mRNA expression was significantly increased (P < 0.05); Number of apoptotic neurons in hippocampus was significantly decreased (P < 0.05). Conclusion RSGB can inhibit inflammatory response and reduce apoptosis of hippocampal neurons, thereby improving the cognitive dysfunction in mice after femoral fracture.

R285.5

中药制药共性技术国家重点实验室开放基金资助项目（202009）