[关键词]
[摘要]
目的 研究艾草Artemisia argyi种子部位的化学成分。方法 利用现代色谱分离技术包括大孔树脂、C18-ODS、Sephadex LH-20及制备型HPLC进行分离纯化,根据NMR、MS等波谱数据及化合物的理化性质鉴定化合物结构。结果 从艾叶种子95%乙醇提取物中分离得到18个化合物,分别鉴定为desacetylmatricarin(1)、3-methoxytanapartholide(2)、3α-chloro- 8α-acetoxy-4β,10α-dihydroxy-1β,2β-epoxy-5α,7αH-guai-11(13)-en-12,6α-olide(3)、1α,6α,8α-trihydroxy-5α,7βH-guaia-3,10(14), 11(13)-trien-12-oic acid(4)、11α,13-二氢魃蒿内酯(5)、魃蒿内酯(6)、tuberiferin(7)、8-acetylrupicolin B(8)、lirioresinol A(9)、丁香树脂酚(10)、dehydroleucodine(11)、leukodin(12)、matricarin(13)、8-acetylrupicolin A(14)、methyl-4S-6α- hydroxy-3-oxoeudesma-1,11(13)-dien-12-oate(15)、滨蒿内酯(16)、异东莨菪素(17)、8-羟基-6,7-二甲氧基香豆素(18)。结论 化合物4为首次从该属植物中分离得到,化合物2、3、5、8~12、14~16、18首次从艾草中分离得到。化合物6~8、11、15在10 µmol/L时对胃癌细胞MGC-803抑制率为61.8%~67.5%,具有显著的抑制活性。
[Key word]
[Abstract]
Objective To study the chemical constituents from the seeds of Artemisia argyi. Methods The chemical constituents from the seeds of A. argyi were isolated and purified by modern chromatographic separation techniques such as macroporous resin, C18-ODS, Sephadex LH-20 and preparative HPLC. The structures were identified by the NMR, MS spectra data and physicochemical properties analysis. Results Eighteen compounds were isolated and identified from 95% ethanol extract from the seeds of A. argyi, and their structures were identified as desacetylmatricarin (1), 3-methoxytanapartholide (2), 3α-chloro-8α- acetoxy-4β,10α-dihydroxy-1β,2β-epoxy-5α,7αH-guai-11(13)-en-12,6α-olide (3), 1α,6α,8α-trihydroxy-5α,7βH-guaia-3,10(14),11(13)- trien-12-oic acid (4), 11α,13-dihydroyomogin (5), yomogin (6), tuberiferin (7), 8-acetylrupicolin B (8), lirioresinol A (9), syringaresinol (10), dehydroleucodine (11), leukodin (12), matricarin (13), 8-acetylrupicolin A (14), methyl-4S-6α- hydroxy-3-oxoeudesma-1,11(13)-dien-12-oate (15), scoparone (16), isoscopoletin (17), and 8-hydroxy-6,7-dimethoxycoumarin (18). Conclusion Compound 4 is separated from the genus of Artemisia for the first time. Compounds 2, 3, 5, 8-12, 14-16 and 18 are isolated from A. argyi for the first time. Cytotoxic effects of compounds 1-18 are investigated in four different gastric cancer cell lines, and the results show that compounds 6-8, 11 and 15 have significant inhibitory activity on MGC-803 cell line at 10 μmol/L with the inhibition rate in the range 61.8%-67.5%.
[中图分类号]
R284.1
[基金项目]
国家自然基金资助项目(82003606);河南省高等学校重点科研项目(21A360002);河南省科技攻关项目(182102310288);河南中医药大学科研苗圃工程项目(MP2020-29);河南省高等学校大学生创新训练计划项目(CXXM[2019]0064)