目的 研究麻疯树Jatropha curcas叶的化学成分及其抗炎活性。方法 采用硅胶、大孔吸附树脂、半制备HPLC等色谱技术进行分离纯化，通过理化性质和NMR、HR-ESI-MS、ECD等现代波谱学技术对化合物的结构进行鉴定；采用Griess法测定化合物对脂多糖（LPS）诱导小鼠巨噬细胞（RAW264.7）释放炎症介质一氧化氮的抑制作用。结果 从麻疯树叶的正丁醇部位中分离得到6个化合物，分别鉴定为 (＋)-jatrointelignan B-4''-O-β-D-glucopyranoside（1）、3-hydroxy-4,7- megastigmadien-3-one 9-O-[α-arabinopyranosyl-(1→6)-β-glucopyranoside]（2）、β-紫罗兰酮-4-O-[β-呋喃芹糖-(1→6)-β-吡喃葡萄糖苷]（3）、byzantionoside B（4）、foliasalacioside B₁（5）、(6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one 9-O-[α-L- arabinopyranosyl-(1→6)-β-D-glucopyranoside]（6）。结论 化合物1～3为新化合物，命名为盐边麻疯苷A～C。化合物4～6为首次从该属植物中分离得到。所有化合物在50.0 μmol/L浓度时，对一氧化氮的产生均无抑制作用。

Objective To study the chemical compositions from leaves of Jatropha curcas and their anti-inflammatory activities. Methods The compounds were isolated and purified by silica gel, HP-20 macroporous resin and semi-preparative HPLC, and their structures were elucidated by physical and spectroscopic analysis. All compounds were tested for inhibitory activities against LPS-induced nitric oxide production in RAW264.7 cells by Griess method. Results Three new compounds, (+)-jatrointelignan B-4''-O-β-D-glucopyranoside (1), 3-hydroxy-4,7-megastigmadien-3-one 9-O-[α-arabinopyranosyl-(1→6)-β-glucopyranoside] (2), β-ionone-4-O-[β-apiofuranosyl-(1→6)]-β-glucopyranoside (3), and three known ones, byzantionoside B (4), foliasalacioside B₁(5), and (6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one 9-O-[α-L-arabinopyranosyl-(1→6)-β-D-glucopyranoside] (6), were identified from n-BuOH extract of J. curcas leaves. Conclusion Compounds 1-3 are new compounds named jatrcaside A-C. Compounds 4-6 are isolated from the Jatropha genus for the first time. All compounds show no inhibition against LPS-induced NO production in a macrophage cell line RAW264.7 at 50.0 μmol/L.

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国家自然科学基金资助项目（81703392）；陕西高校青年创新团队