[关键词]
[摘要]
目的 对甘遂Euphorbia kansui块根中的三萜化学成分和抗氧化活性进行研究。方法 采用多种柱色谱法进行分离纯化，并运用谱学分析鉴定化合物的结构。通过CCK-8法，对分离的化合物在SH-SY5Y细胞中的抗氧化活性进行了筛选。结果 从甘遂块根甲醇提取物中分离鉴定出5个三萜，其中包括3个大戟烷型，分别为大戟-8,24-二烯-3β-醇-7,11-二酮（1）、(24R)-大戟-8,25-二烯-3β,24-二醇（2）、大戟二烯醇（3）；2个羊毛脂烷型三萜，3β,11β-二羟基羊毛脂-8,24-二烯-7-酮（4）、3β,7β-二羟基羊毛脂-8,24-二烯-11-酮（5）。结论 化合物5为新化合物，为羊毛脂烷型三萜，命名为甘遂羊毛脂酮A；化合物1和4为首次从该植物中分离得到；化合物2和5在SH-SY5Y细胞中具有一定的抗氧化能力和潜在的神经保护活性。
[Key word]
[Abstract]
Objective To isolate compounds from the roots of Euphorbia kansui and investigate their antioxidant activities. Methods Various column chromatography techniques were used to isolate and purify the compounds and their structures were identified by spectral data. Meanwhile, the isolated compounds were evaluated for antioxidant activity in SH-SY5Y cells. Results Five triterpenes were isolated and identified from the methanol extract of the roots of E. kansui, including three euphane triterpenes, namely eupha-8,24-diene-3β-ol-7,11-dione(1), (24R)-eupha-8,25-diene-3β,24-diol (2), euphol (3), and two lanostane triterpenes namely 3β,11β-dihydroxylanosta-8,24-dien-7-one (4), 3β,7β-dihydroxylanosta-8,24-dien-11-one(5). Conclusion Compound 5 is a new compound named euphokansuione A and compounds 1 and 4 are isolated from this plant for the first time. Compounds 2 and 5 have certain antioxidant capacity in SH-SY5Y cells and potential neuroprotective activity.
[中图分类号]
R284.1
[基金项目]
甘肃省自然科学基金项目（21JR7RA075）；甘肃省自然科学基金项目（20JR5RA570）
