目的 基于HPLC指纹图谱与多成分含量测定，结合化学模式识别法评价胡桃楸Juglans mandshurica叶质量，为其深入研究和开发提供依据。方法 采用HPLC法建立30批不同产地胡桃楸叶指纹图谱；通过中药色谱指纹图谱相似度评价系统（2012版）进行相似度评价；通过对照品比对指认了3种化学成分，并测定了样品中3种成分的含量；使用SPSS 25.0软件进行聚类分析、主成分分析和相关性分析。结果 30批胡桃楸叶指纹图谱共标定了12个共有峰，指认的3个共有峰为绿原酸、金丝桃苷和异槲皮苷，指纹图谱相似度范围是0.827～0.995，具有较好的一致性；样品中绿原酸、金丝桃苷和异槲皮苷3种化学成分的质量分数分别为0.258～5.607 mg/g、0.113～3.974 mg/g和0.439～6.537 mg/g；聚类分析将样品分为3类，30批样品中28批样品聚为一类，铁岭鸡冠山乡（S3）、本溪东营坊乡（S6）的样品各自聚为一类；主成分分析用2个主成分进行综合评价，综合得分大于1的前6位分别为S3、S6、S12、S8、S24和S18；30批样品中3种成分的总含量与主成分综合得分存在明显相关性。结论 建立的胡桃楸叶质量评价方法操作简便、结果可靠，绿原酸、金丝桃苷和异槲皮苷可以作为胡桃楸叶质量评价的指标性成分。

Objective To evaluate the quality of leaves of Juglans mandshurica based on HPLC fingerprint and multi-component determination combined with chemical pattern recognition method for providing useful information for further study and utilization. Methods The fingerprints of 30 batches of leaves of J. mandshurica from different producing areas were established by HPLC, and the similarity of the fingerprints was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia (2012). Three kinds of components were identified by comparison with reference substances and their content in samples were determined. The hierarchical clustering analysis (HCA), principal component analysis (PCA) and correlation analysis (CA) were performed by SPSS 25.0. Results Twelve common peaks were identified in 30 batches of leaves of J. mandshurica. Three of the common peaks were identified as chlorogenic acid, hyperoside and isoquercitrin. The fingerprint similarities ranged from 0.827 to 0.995 with good consistency. The qulity score of chlorogenic acid, hyperoside and isoquercitrin in samples were 0.258—5.607 mg/g, 0.113—3.974 mg/g, and 0.439—6.537 mg/g, respectively. According to HCA, the samples were divided into three groups. A total of 28 of 30 batches were clustered into one group, and samples from Jiguan Town of Tieling City (S3) and Dongyingfang Town of Benxi City (S6) were clustered as one group respectively. Two principal components were selected and the comprehensive scores of each sample were achieved by PCA. The results of PCA showed that the top six comprehensive scores of S3, S6, S12, S8, S24 and S18 were bigger than 1. There was a significant correlation between the total content and the comprehensive scores of three components in the 30 batches of leaves of J. mandshurica. Conclusion The established method is simple to operate and reliable, the total content of chlorogenic acid, hyperoside and isoquercitrin can be used as an marker for the quality evaluation of leaves of J. mandshurica.

R286.2

国家自然科学基金项目(81703658)