目的 基于超分子传感技术研究血府逐瘀汤对三甲胺结肠吸收的影响。方法 采用离体外翻肠囊模型研究三甲胺的结肠吸收;基于指示剂置换分析策略,构建选择性识别三甲胺的超分子荧光传感体系,评价血府逐瘀汤对三甲胺结肠吸收的影响。结果 基于杯芳烃分子识别的超分子传感体系实现了三甲胺的灵敏检测;与对照组相比,血府逐瘀汤高剂量(5.0 mg/mL)组显著降低结肠单位面积三甲胺的累积吸收量(P<0.05、0.01、0.001),血府逐瘀汤低剂量(2.5 mg/mL)组能够减少结肠单位面积三甲胺的累积吸收量,但与对照组相比无显著性差异。结论 血府逐瘀汤能够减少三甲胺的结肠吸收,为血府逐瘀汤活血化瘀作用提供一定依据。

Objective To study the effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on trimethylamine (TMA) absorption based on supramolecular sensing technology. Methods The model of everted intestinal sac was used to explore the affection of Xuefu Zhuyu Decoction on colon absorption of TMA. Based on the indicator displacement assay, a supramolecular fluorescence sensing system was established for selective recognition of TMA, which was employed to evaluate the effect of Xuefu Zhuyu Decoction on colonic absorption of TMA. Results The established supramolecular sensing method can successfully detect TMA via molecular recognition of calixarene. Compared with control group, the cumulative absorption of TMA per unit area of colon in high-dose Xuefu Zhuyu Decoction (5.0 mg/mL) group was significantly decreased (P < 0.05, 0.01, 0.001), and there was no significant difference in low-dose Xuefu Zhuyu Decoction (2.5 mg/mL) group. Conclusion Xuefu Zhuyu Decoction can reduce colonic absorption of TMA, which provides the basis for the role of Xuefu Zhuyu Decoction in promoting blood circulation and removing blood stasis.

R285.5

国家自然科学基金资助项目（81873192）