目的 采用代谢组学方法分析人参固本口服液(Ginseng Guben Oral Liquid,RSGB)对食蟹猴人工颈椎间盘置换术后血清代谢产物的影响,探讨人参固本口服液促进猕猴颈椎术后康复的机制。方法 24只食蟹猴根据体质量随机分为正常组、模型组、RSGB组,每组8只。通过食蟹猴人工颈椎间盘置换术制备模型,正常组、模型组ig给予蒸馏水,RSGB组ig给予人参固本口服液,观察食蟹猴术后康复情况。运用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术(UHPLC-ESI-QE-Orbitrap-MS)检测血清差异代谢物,分析可能的代谢通路。结果 RSGB组食蟹猴一般情况明显改善。代谢组学显示,与正常组相比,模型组食蟹猴结肠共发现乙酰胆碱、烟酰胺、蛋氨酸、乙酰左旋肉碱、次黄嘌呤、鸟嘌呤、肌苷、鸟苷、次黄嘌呤、亮氨酸、异喹啉、脱氧胆酸、胆酸、肉豆蔻酸、棕榈油酸、二十二碳六烯酸、亚油酸、花生四烯酸、肉豆蔻酸乙酯、油酸等23个代谢差异物。RSGB组这些代谢差异物有向正常水平回调的趋势。结论 推测RSGB促进食蟹猴颈椎术后康复可能与调控亚油酸代谢、花生四烯酸代谢、色氨酸及半胱氨酸和蛋氨酸代谢等多条代谢通路有关。

Objective Metabolomics method was used to analyze the effects of Ginseng Guben Oral Liquid (人参固本口服液, RSGB) on serum metabolites in cynomophagous monkeys after cervical disc replacement. To explore the mechanism of RSGB promoting cervical vertebra postoperative rehabilitation in cynomophagous monkeys. Methods According to body weight, 24 cynomophagous monkeys were randomly divided into normal group, model group and RSGB group, with eight monkeys in each group. The models were made by cervical disc replacement in cynomophagous monkeys, normal group and model group were given distilled water intragastrically, RSGB group was given RSGB intragastrically, and the postoperative rehabilitation of cynomolgus monkeys was observed. Ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry (UHPLC-ESI-Orbitrap-MS/MS) was used to detect differential metabolites in blood and analyze possible metabolic pathways. Results The results showed that the general condition of cynomophagous monkeys in RSGB group was significantly improved. Metabonomics showed that compared with the normal group, model group were found colon acetylcholine, niacinamide, methionine, acetyl-L-carnitine, inosine, guanosine, hypoxanthine, leucine, isoquinoline, deoxycholic acid, cholic acid, myristic acid, palmitoleic acid, docosahexaenoic acid, linoleic acid, arachidonic acid, ethyl myristate, oleic acid and other 23 metabolic differentials. In the RSGB group, these metabolic differentials tended to return to the normal level. Conclusion It is speculated that the promotion of RSGB in postoperative rehabilitation of cervical vertebra in cynomophagous monkeys may be related to the regulation of linoleic acid metabolism, arachidonic acid metabolism, tryptophan, cysteine and methionine metabolism and other metabolic pathways.

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国家科技重大专项（2018ZX09201010）；广东省医学科研基金（A2019217）；江门市科技计划项目（2021YL03002）