目的 对荆防颗粒化学成分进行系统分析,并分析其裂解规律。方法 采用GC-MS技术对荆防颗粒中挥发性化学成分和UPLC-Q-Exactive MS技术在正、负离子模式下对荆防颗粒中非挥发性化学成分进行定性分析。结果 通过GC-MS数据库匹配,共鉴别出了24个化合物,包括萜类19个、醛类2个、酯类2个、芳香醚类1个。通过质谱信息并结合对照品、相关文献、数据库检索,液质联用共鉴别出了85个化合物,包括香豆素类25个、木脂素类6个、黄酮苷15个、黄酮类15个、有机酸类9个、糖苷类5个、三萜类4个和其他类化合物6个。结论 建立了荆防颗粒的GC-MS和UPLC-Q-Exactive MS的分离鉴定方法,为阐释该复方的功效物质基础提供了依据。

Objective To investigate the chemical composition of Jingfang Ganules (荆防颗粒), and analyze its fragmentation pattern. Methods The volatile and non-volatile chemical components in Jingfang Granules were qualitatively analyzed by GC-MS and UPLC-Q-Exactive MS in positive and negative ion mode, respectively. Results A total of 24 compounds (19 terpenoids, two aldehydes, two esters and one aromatic ether) were identified by GC-MS. And a total of 85 compounds, (25 coumarins, six lignans, 15 flavonoid glycosides, 15 flavonoids, nine organic acids, five glycosides, four triterpenoids and six other compounds) were identified by UPLC-Q Exactive MS and combined with reference materials, related literatures and database retrieval. Conclusion The separation and identification methods of Jingfang Granules by GC-MS and UPLC-Q Exactive MS were established, which provided a foundation for further research of pharmacodynamic material basis of it.

R284.1

国家重大新药创制专项（2017ZX09301058）