目的 建立不同来源竹茹药材HPLC指纹图谱，结合相似度、聚类、主成分分析等化学计量手段评价不同产地竹茹药材品质，为其质量控制研究提供技术方法借鉴和基础数据。方法 通过HPLC梯度洗脱建立23个批次竹茹药材的指纹图谱，采用中药色谱指纹图谱相似度评价系统（2012A版）对竹茹药材HPLC指纹图谱进行相似度评价，并采用SPSS24.0软件进行聚类、主成分分析。结果 选取了13个色谱共有峰，23批样品共有峰相似度在0.713～0.991；聚类分析将23批样品分为2类；主成分和因子分析筛选出累积方差达77.83%贡献率的3个主成分，能够反映23个批次竹茹药材特征性质量差异。结论 建立分析的HPLC指纹图谱结合化学计量学评价方法，简便可行，可为综合评价不同产地竹茹药材的质量提供参考。

Objective To establish the fingerprints of Caulis Bambusae In Taeniam from different habitats by HPLC, and comprehensively analyze the quality of C. Bambusae In Taeniam by the similarity evaluation in combination with principal component, clustering, principal component analysis (PCA) and other chemo-metrics, so as to provide theoretical basis for its quality evaluation. Methods The fingerprints of 23 batches of C. Bambusae In Taeniam were established by HPLC gradient elution, and the similarity evaluation of the HPLC fingerprints of C. Bambusae In Taeniam medicinal materials was carried out using the traditional Chinese medicine chromatographic fingerprint similarity evaluation system (2012A version), and SPSS24.0 software was used for clustering and PCA analysis. Results Thirteen chromatographic peaks were selected as the common peaks, and the similarity of the 23 batches was 0.713—0.991. Principal components and PCA analysis screened out three principal components with cumulative variance contribution rate of 77.83%, reflecting the quality characteristic differences of 23 batches of C. Bambusae In Taeniam medicinal materials. Conclusion HPLC fingerprint established in this experiment combined with the chemometric evaluation method is simple, feasible and reproducible, and can be used to comprehensively evaluate the quality of C. Bambusae In Taeniam from different producing areas.

R286.2

江苏省研究生科研与实践创新计划项目（SJCX20_0560，SJCX20_0563）