目的 探析并比较野生甘松Nardostachys jatamansi与仿野生栽培甘松的根际细菌与内生细菌的种群组成、丰富度、多样性及差异性。方法 以野生甘松和仿野生栽培甘松的根际土壤、根及根茎为研究样本，基于Illumina MiSeq第二代高通量测序技术对根际土壤细菌、根及根茎内生细菌的16S rDNA V4-V5区进行序列测定，并进行相关生物信息学分析。结果 各组样本基因组经16S测序，细菌群落的所有序列分别进行97%的相似水平下的物种分类单元（operational taxonomic units，OUT）分类，根际细菌共涉及22门、38纲、69目、119科、254属、268种，内生细菌共涉及16门、29纲、60目、107科、199属、145种。根际、内生细菌群落中共有的序列分别为498个、177个，主成分分析的最主要2类主成分（PC1和PC2）类群的比例综合分别接近70%、60%，其中丰度排在前4位的依次为变形菌门、放线菌门、酸杆菌门和拟杆菌门，其他类群的类别及相对丰度存在一定差异。优势细菌差异分析中，各组细菌群落组成及相对丰度差异很大。结论 野生甘松和仿野生栽培甘松的优势促生菌群可能主要集中于变形菌门、放线菌门、酸杆菌门和拟杆菌门，可从中筛选出核心功能微生物混合菌群，对改良仿野生培育甘松土壤的微生物环境具有理论指导意义。

Objective To analyze and compare the population composition, richness, diversity and difference in rhizosphere and endophytic bacteria of the wild and wild imitating cultivated Nardostachys jatamansi. Methods The 16S rDNA V4-V5 region of rhizosphere bacteria in soil and endophytic bacteria in roots and rhizomes of the wild and wild imitating cultivated N. jatamansi was sequenced by using the Illumina Miseq second-generation high-throughput sequencing technology, and the related biological information was also analyzed. Results The genome of each sample was sequenced by 16S, and all sequences of bacterial community were OUT classified at 97% similar level, respectively. A total of 22 phyla, 38 classes, 69 orders, 119 families, 254 genera, and 268 species were detected in the rhizosphere bacteria of N. jatamansi. And there were 16 phyla, 29 classes, 60 orders, 107 families, 199 genera, and 145 species in rhizosphere bacteria of N. jatamansi. The common sequences of rhizosphere and endophytic bacterial communities were 498 and 177, respectively. The comprehensive proportions of PC1 and PC2 groups in principal component analysis were close to 70% and 60%, respectively. Among them, Proteobacteria, Actinobacteria, Acidobacteria and Bacteroidetes ranked the top four in the order of abundance, while there were some differences in the categories and relative abundance of other groups. Conclusion The main growthpromoting phylums of wild and wild imitating cultivated N. jatamansi. may be mainly concentrated in Proteobacteria, Actinobacteria, Acidobacteria and Bacteroidetes, from which the core functional microorganism mixed flora can be screened out. These results can play a theoretical guiding significance for improving the soil microbial community of wild imitating cultivated N. jatamansi.

R282.2

国家重点研发计划（2018YFC1706101）；四川省科技计划应用基础项目（2020YJ0099）；中央高校基本科研业务费专项资金研究类项目（2018NQN13）